ArticleScientific reports2015
Differentiation of early germ cells from human skin-derived stem cells without exogenous gene integration.
Article in Scientific reports, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 35 citations in OpenAlex.
- Transcriptomic landscape reveals germline potential of porcine skin-derived multipotent dermal fibroblast progenitors.Cellular and molecular life sciences : CMLS · 2023Article
- Murine skin-derived multipotent papillary dermal fibroblast progenitors show germline potential in vitro.Stem cell research & therapy · 2023Article
- iPSCs derived from infertile men carrying complex genetic abnormalities can generate primordial germ-like cells.Scientific reports · 2022Article
- The proliferation role of LH on porcine primordial germ cell-like cells (pPGCLCs) through ceRNA network construction.Clinical and translational medicine · 2021Article
- Production of viable chicken by allogeneic transplantation of primordial germ cells induced from somatic cells.Nature communications · 2021Article
- Dissecting the initiation of female meiosis in the mouse at single-cell resolution.Cellular and molecular life sciences : CMLS · 2021Article
- Research Progress on the Treatment of Premature Ovarian Failure Using Mesenchymal Stem Cells: A Literature Review.Frontiers in cell and developmental biology · 2021Review
- In Vitro Generation of Oocyte Like Cells and Their In Vivo Efficacy: How Far We have been Succeeded.Cells · 2020Review
- Article
- Retinoic acid enhances germ cell differentiation of mouse skin-derived stem cells.Journal of ovarian research · 2018Article
- Making gametes from alternate sources of stem cells: past, present and future.Reproductive biology and endocrinology : RB&E · 2017Review
- Review
- Skin-derived stem cells as a source of primordial germ cell- and oocyte-like cells.Cell death & disease · 2016Review
- Human somatic cells subjected to genetic induction with six germ line-related factors display meiotic germ cell-like features.Scientific reports · 2016Article
Corrections and comments
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Authors and funding
11 authors at 4 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Infertility has long been a difficult issue for many couples. The successful differentiation of germ cells and live progeny from pluripotent stem cells brings new hope to the couples suffering with infertility. Here we successfully isolated human fetus skin-derived stem cells (hfSDSCs) from fetus skin tissue and demonstrated that hfSDSCs can be differentiated into early human germ cell-like cells (hGCLCs). These cells express human germ cell markers DAZL and VASA. Moreover, these pluripotent stem cell-derived hGCLCs are free of exogenous gene integration. When hfSDSCs were differentiated in porcine follicle fluid (PFF) conditioned media, which has been shown to promote the differentiation of mouse and porcine SDSCs into oocyte-like cells (OLCs), we observed some vesicular structures formed from hfSDSCs. Moreover, when hfSDSCs were cultured with specific conditioned media, we observed punctate and elongated SCP3 staining foci, indicating the initiation of meiosis. Ploidy analysis and fluorescent in situ hybridization (FISH) analysis indicated that a small percentage of putative 1N populations formed from hfSDSCs when compared with positive controls. In conclusion, our data here, for the first time, demonstrated that hfSDSCs possess the differentiation potential into germ lines, and they may differentiate both male and female hGCLCs in vitro under appropriate conditions.
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