Evidence map›Paper›PMID 26347365›Full record

ArticleScientific reports2015

Functionalized Buckyballs for Visualizing Microbial Species in Different States and Environments.

Qingsu Cheng, Ashwin Aravind, Matthew Buckley, Andrew Gifford, Bahram Parvin

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Scientific reports, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Qingsu ChengDepartment of Electrical and Biomedical Engineering, University of Nevada, Reno, 1664 N Virginia Street, Reno NV, 89503, USA.
Ashwin AravindLife Sciences Division, Lawrence Berkeley National Laboratory, 1 Cyclotron Road, MS-977, Berkeley CA, 94720, USA.
Matthew BuckleyLife Sciences Division, Lawrence Berkeley National Laboratory, 1 Cyclotron Road, MS-977, Berkeley CA, 94720, USA.
Andrew GiffordBrookhaven National Laboratory, Bioscience Department, Bldg490, Upton, NY 11973, USA.
Bahram ParvinDepartment of Electrical and Biomedical Engineering, University of Nevada, Reno, 1664 N Virginia Street, Reno NV, 89503, USA.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

To date, in situ visualization of microbial density has remained an open problem. Here, functionalized buckyballs (e.g., C60-pyrrolidine tris acid) are shown to be a versatile platform that allows internalization within a microorganism without either adhering to the cell wall and cell membrane or binding to a matrix substrate such as soil. These molecular probes are validated via multi-scale imaging, to show association with microorganisms via fluorescence microscopy, positive cellular uptake via electron microscopy, and non-specific binding to the substrates through a combination of fluorescence and autoradiography imaging. We also demonstrate that cysteine-functionalized C60-pyrrolidine tris acid can differentiate live and dead microorganisms.

Indexed as

BiodiversitySoil MicrobiologyFullerenesMicroscopy, ConfocalMicroscopy, ElectronFullerenes

Identifiers

PMID26347365
PMCPMC4561912

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.