ArticleScientific data2015
A resource from 3D electron microscopy of hippocampal neuropil for user training and tool development.
Article in Scientific data, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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Who cites it
30 citing papers in PubMed, 53 citations in OpenAlex.
- Curvature-based machine-learning method for automated segmentation of dendritic spines.Biophysical journal · 2026Article
- Presynaptic Vesicles Supply Membrane for Axonal Bouton Enlargement during Long Term Potentiation.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2026Article
- Ultrastructure of astrocytes using volume electron microscopy: A scoping review.The Journal of physiology · 2026Article
- CURVATURE-BASED MACHINE LEARNING METHOD FOR AUTOMATED SEGMENTATION OF DENDRITIC SPINES.bioRxiv : the preprint server for biology · 2025Article
- Perisynaptic Astroglial Response to In Vivo Long-Term Potentiation and Concurrent Long-Term Depression in the Hippocampal Dentate Gyrus.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2025Article
- Mitochondria structurally remodel near synapses to fuel the sustained energy demands of plasticity.bioRxiv : the preprint server for biology · 2025Article
- PyReconstruct: A fully open-source, collaborative successor to Reconstruct.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Ultrafast endocytosis in mouse cortical inhibitory synapses.bioRxiv : the preprint server for biology · 2025Article
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- Sparse Annotation is Sufficient for Bootstrapping Dense Segmentation.Research square · 2024Article
- Sparse Annotation is Sufficient for Bootstrapping Dense Segmentation.bioRxiv : the preprint server for biology · 2024Article
- Long-Term Potentiation Produces a Sustained Expansion of Synaptic Information Storage Capacity in Adult Rat Hippocampus.bioRxiv : the preprint server for biology · 2024Article
- Unambiguous identification of asymmetric and symmetric synapses using volume electron microscopy.Frontiers in neuroanatomy · 2024Article
- Excitatory and inhibitory imbalances in the trisynaptic pathway in the hippocampus in schizophrenia: a postmortem ultrastructural study.Journal of neural transmission (Vienna, Austria : 1996) · 2023Article
- Joint reconstruction of neuron and ultrastructure via connectivity consensus in electron microscope volumes.BMC bioinformatics · 2022Article
- Article
- Dendritic Spine Density Scales with Microtubule Number in Rat Hippocampal Dendrites.Neuroscience · 2022Article
- Unique properties of dually innervated dendritic spines in pyramidal neurons of the somatosensory cortex uncovered by 3D correlative light and electron microscopy.PLoS biology · 2021Article
- Neural anatomy and optical microscopy (NAOMi) simulation for evaluating calcium imaging methods.Journal of neuroscience methods · 2021Article
- Nanoscale 3D EM reconstructions reveal intrinsic mechanisms of structural diversity of chemical synapses.Cell reports · 2021Article
Corrections and comments
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Authors and funding
8 authors at 4 institutions in 2 countries.
Funding
Abstract
Resurgent interest in synaptic circuitry and plasticity has emphasized the importance of 3D reconstruction from serial section electron microscopy (3DEM). Three volumes of hippocampal CA1 neuropil from adult rat were imaged at X-Y resolution of ~2 nm on serial sections of ~50-60 nm thickness. These are the first densely reconstructed hippocampal volumes. All axons, dendrites, glia, and synapses were reconstructed in a cube (~10 μm(3)) surrounding a large dendritic spine, a cylinder (~43 μm(3)) surrounding an oblique dendritic segment (3.4 μm long), and a parallelepiped (~178 μm(3)) surrounding an apical dendritic segment (4.9 μm long). The data provide standards for identifying ultrastructural objects in 3DEM, realistic reconstructions for modeling biophysical properties of synaptic transmission, and a test bed for enhancing reconstruction tools. Representative synapses are quantified from varying section planes, and microtubules, polyribosomes, smooth endoplasmic reticulum, and endosomes are identified and reconstructed in a subset of dendrites. The original images, traces, and Reconstruct software and files are freely available and visualized at the Open Connectome Project (Data Citation 1).
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.