Evidence map›Paper›PMID 26347123›Full record

ArticleZeitschrift fur Rheumatologie2015

JHDM1D and HDAC1-3 mRNA expression levels in peripheral blood mononuclear cells of patients with systemic lupus erythematosus.

M J Nawrocki, A J Strugała, P Piotrowski, M Wudarski, M Olesińska, P P Jagodziński

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Article in Zeitschrift fur Rheumatologie, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
0.3field-weighted citation impact, top 42% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

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3 · Its place in the literature

Who cites it

6 citing papers in PubMed, 17 citations in OpenAlex.

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4 · The record

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5 · Who and what money

Authors and funding

6 authors at 5 institutions in 1 country.

M J NawrockiDepartment of Biochemistry and Molecular Biology, Poznań University of Medical Sciences, 6 Święcickiego St, 60-781, Poznań, Poland. mjnawrocki@ump.edu.pl.
A J StrugałaDepartment of Molecular and Systems Biology, Institute of Bioorganic Chemistry Polish Academy of Sciences, Poznań, Poland.
P PiotrowskiDepartment of Biochemistry and Molecular Biology, Poznań University of Medical Sciences, 6 Święcickiego St, 60-781, Poznań, Poland.
M WudarskiInstitute of Rheumatology, Warsaw, Poland.
M OlesińskaInstitute of Rheumatology, Warsaw, Poland.
P P JagodzińskiDepartment of Biochemistry and Molecular Biology, Poznań University of Medical Sciences, 6 Święcickiego St, 60-781, Poznań, Poland.
National Institute of Geriatrics, Rheumatology and Rehabilitation · PLHeliodor Swiecicki Clinical Hospital · PLInstitute of Bioorganic Chemistry, Polish Academy of Sciences · PLMossakowski Medical Research Institute, Polish Academy of Sciences · PLPoznan University of Medical Sciences · PL

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundSystemic lupus erythematosus (SLE) is a chronic relapsing autoimmune disease characterized by production of autoantibodies against a series of nuclear antigens and by chronic inflammation. The etiology of SLE is the result of interactions between genetic, epigenetic, hormonal, and environmental factors. Changes in histone acetylation and methylation contribute to structural chromatin modifications.

objectiveWe studied the histone demethylase JHDM1D and histone deacetylases HDAC1, HDAC2, and HDAC3 transcript levels in peripheral blood mononuclear cells (PBMCs) from patients diagnosed with systemic lupus erythematosus (SLE). Furthermore, the association of JHDM1D, HDAC1, HDAC2, and HDAC3 transcript levels with gender, age, and major clinical manifestations were analyzed. MATERIALS AND

methodsReal-time quantitative polymerase chain reaction (RQ-PCR) analysis was used to determine JHDM1D, HDAC1, HDAC2, and HDAC3 mRNA expression levels in peripheral blood mononuclear cells (PBMCs) from 30 patients with SLE and 36 healthy controls.

resultsSignificantly lower HDAC2 transcript levels (p = 0.006785) and significantly higher JHDM1D (p = 0.0000002) and HDAC1 (p = 0.010581) transcript levels in SLE patients were observed compared with healthy controls. Higher JHDM1D mRNA expression was detected in active SLE patients when compared with inactive patients (p = 0.005). Furthermore, the JHDM1D transcript levels were positively correlated with disease activity (r(s) = 0.368, p = 0.045), while HDAC2 mRNA expression was positively correlated with disease duration (r(s) = 0.502, p = 0.0047).

conclusionOur analyses confirmed the importance of epigenetic alterations (histone demethylation and acetylation) in SLE etiology. Moreover, our results suggest that the presence of some clinical manifestations, like hematological disease and anti-Ro antibody, might be associated with the dysregulation of histone demethylase and deacetylases mRNA expression levels.

Indexed as

AdultBiomarkersFemaleGenetic Predisposition to DiseaseHistone DeacetylasesHumansJumonji Domain-Containing Histone DemethylasesLeukocytes, MononuclearLupus Erythematosus, SystemicMaleReproducibility of ResultsRNA, MessengerSensitivity and SpecificityBiomarkersHistone DeacetylasesJumonji Domain-Containing Histone DemethylasesKDM7A protein, humanRNA, MessengerAntigens, nuclearEpigenesis, geneticHistone deacetylasesHistone demethylasesHistone modifications

Identifiers

PMID26347123
OpenAlexW1478750466

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.