ArticleScientific reports2015
Pathway optimization by re-design of untranslated regions for L-tyrosine production in Escherichia coli.
Article in Scientific reports, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
22 citing papers in PubMed, 59 citations in OpenAlex.
- Metabolic Engineering for Biotechnological Production of Bioactive Methylated Flavonoids and Stilbenes: Progress and Perspectives.Applied biochemistry and biotechnology · 2026Review
- The knowledge driven DBTL cycle provides mechanistic insights while optimising dopamine production in Escherichia coli.Microbial cell factories · 2025Article
- Metabolic engineering of Corynebacterium crenatum for enhanced L-tyrosine production from mannitol and glucose.Microbial cell factories · 2024Article
- Blended nexus molecules promote COScience advances · 2024Article
- Recent Advances in Metabolic Engineering for the Biosynthesis of Phosphoenol Pyruvate-Oxaloacetate-Pyruvate-Derived Amino Acids.Molecules (Basel, Switzerland) · 2024Review
- An Efficient Prephenate Dehydrogenase Gene for the Biosynthesis of L-tyrosine: Gene Mining, Sequence Analysis, and Expression Optimization.Foods (Basel, Switzerland) · 2023Article
- Engineered Escherichia coli platforms for tyrosine-derivative production from phenylalanine using phenylalanine hydroxylase and tetrahydrobiopterin-regeneration system.Biotechnology for biofuels and bioproducts · 2023Article
- Synthetic small regulatory RNAs in microbial metabolic engineering.Applied microbiology and biotechnology · 2021Review
- Simultaneous Multiplex Genome EngineeringFrontiers in microbiology · 2021Article
- A bi-enzymatic cascade to yield pyruvate as co-substrate for L-tyrosine production.Applied microbiology and biotechnology · 2020Article
- Microbial Upgrading of Acetate into Value-Added Products-Examining Microbial Diversity, Bioenergetic Constraints and Metabolic Engineering Approaches.International journal of molecular sciences · 2020Review
- Chromosome Engineering To Generate Plasmid-Free Phenylalanine- and Tyrosine-OverproducingApplied and environmental microbiology · 2020Article
- Metabolic engineering of Escherichia coli for production of chemicals derived from the shikimate pathway.Journal of industrial microbiology & biotechnology · 2020Review
- Upcycling chitin-containing waste into organonitrogen chemicals via an integrated process.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- Recent advances in genetic engineering tools based on synthetic biology.Journal of microbiology (Seoul, Korea) · 2020Review
- CRISPR/Cas9-facilitated engineering with growth-coupled and sensor-guidedMetabolic engineering communications · 2019Article
- Precise tuning of the glyoxylate cycle in Escherichia coli for efficient tyrosine production from acetate.Microbial cell factories · 2019Article
- Production of methylparaben in Escherichia coli.Journal of industrial microbiology & biotechnology · 2019Article
- Chromosome engineering of Escherichia coli for constitutive production of salvianic acid A.Microbial cell factories · 2017Article
- Metabolic engineering of indole pyruvic acid biosynthesis in Escherichia coli with tdiD.Microbial cell factories · 2017Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
L-tyrosine is a commercially important compound in the food, pharmaceutical, chemical, and cosmetic industries. Although several attempts have been made to improve L-tyrosine production, translation-level expression control and carbon flux rebalancing around phosphoenolpyruvate (PEP) node still remain to be achieved for optimizing the pathway. Here, we demonstrate pathway optimization by altering gene expression levels for L-tyrosine production in Escherichia coli. To optimize the L-tyrosine biosynthetic pathway, a synthetic constitutive promoter and a synthetic 5'-untranslated region (5'-UTR) were introduced for each gene of interest to allow for control at both transcription and translation levels. Carbon flux rebalancing was achieved by controlling the expression level of PEP synthetase using UTR Designer. The L-tyrosine productivity of the engineered E. coli strain was increased through pathway optimization resulting in 3.0 g/L of L-tyrosine titer, 0.0354 g L-tyrosine/h/g DCW of productivity, and 0.102 g L-tyrosine/g glucose yield. Thus, this work demonstrates that pathway optimization by 5'-UTR redesign is an effective strategy for the development of efficient L-tyrosine-producing bacteria.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.