Evidence map›Paper›PMID 26346631›Full record

ArticleThe journal of sexual medicine2015

Subacute Hemolysis in Sickle Cell Mice Causes Priapism Secondary to NO Imbalance and PDE5 Dysregulation.

Nikolai A Sopko, Hotaka Matsui, Johanna L Hannan, Dan Berkowitz, Hunter C Champion, Lewis L Hsu, Biljana Musicki, Arthur L Burnett, Trinity J Bivalacqua

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in The journal of sexual medicine, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
9citing papers in PubMed, 1 pooled it
1.3field-weighted citation impact, top 21% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

9 citing papers in PubMed, 1 synthesis or guideline pooled it, 23 citations in OpenAlex.

  1. Pooled it
  2. Review
  3. Review
  4. Review
  5. Resolution of Acute Priapism in Two Children With Sickle Cell Disease Who Received Nitrous Oxide.Academic emergency medicine : official journal of the Society for Academic Emergency Medicine · 2019
    Article
  6. Article
  7. Article
  8. Advances in the understanding of priapism.Translational andrology and urology · 2017
    Review
  9. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 5 institutions in 1 country.

Nikolai A SopkoThe James Buchanan Brady Urological Institute and Department of Urology, The Johns Hopkins School of Medicine, Baltimore, MD, USA.ORCID http://orcid.org/0000-0003-4913-0570
Hotaka MatsuiThe James Buchanan Brady Urological Institute and Department of Urology, The Johns Hopkins School of Medicine, Baltimore, MD, USA.
Johanna L HannanThe James Buchanan Brady Urological Institute and Department of Urology, The Johns Hopkins School of Medicine, Baltimore, MD, USA.
Dan BerkowitzDepartment of Anesthesiology and Critical Care Medicine, and Biomedical Engineering, Johns Hopkins Medical Institutions, Baltimore, MD, USA.
Hunter C ChampionDepartment of Medicine, University of Pittsburgh, Pittsburgh, PA, USA.
Lewis L HsuDepartment of Pediatrics, University of Illinois, Chicago, IL, USA.
Biljana MusickiThe James Buchanan Brady Urological Institute and Department of Urology, The Johns Hopkins School of Medicine, Baltimore, MD, USA.
Arthur L BurnettThe James Buchanan Brady Urological Institute and Department of Urology, The Johns Hopkins School of Medicine, Baltimore, MD, USA.
Trinity J BivalacquaThe James Buchanan Brady Urological Institute and Department of Urology, The Johns Hopkins School of Medicine, Baltimore, MD, USA.
American Urological Association · USJohns Hopkins University · USUniversity of Illinois Chicago · USUniversity of Maryland, Baltimore · USUniversity of Pittsburgh · US

Funding

Roles of RhoA/Rho-kinase Signaling in Cavernous Neurons Following Axonal InjuryK08DK090370 · NIDDK · JOHNS HOPKINS UNIVERSITY · PI BIVALACQUA, TRINITY JUDE · 2011 to 2015
$794k
NIDDK NIH HHS K08 DK090370
6 · The paper itself

Abstract

introductionRecent research suggests that priapism in sickle cell disease (SCD) is due to dysregulation of penile erection homeostasis including alteration of nitric oxide synthase (NOS) and phosphodiesterase type 5 (PDE5) activities by excessive levels of reactive oxygen species (ROS) released during hemolysis. It is unknown if subacute exposure to hemolysis is sufficient or if chronic reconditioning of erectile tissues is required for perturbation of homeostatic pathways and whether PDE5 inhibitor (PDE5I) treatment can restore erectile homeostasis in the subacute setting.

aimsThe aim of this study was to investigate the effects of subacute hemolysis (3-month exposure) on priapism and NO pathway regulation.

methodsMice underwent bone marrow transplantation with either SCD (BM-SS) or wild-type (WT) bone marrow. BM-SS mice were treated with sildenafil 100 mg/kg/day. We measured intracavernous pressure (ICP) measurements with or without cavernous nerve stimulation following bone marrow transplantation to assess for priapism.

main outcome measuresICP and frequency of erections were assessed. Penile tissues were analyzed for NOS, protein kinase G (PKG), PDE5, and ROS activities.

resultsBM-SS mice demonstrated a priapism phenotype. PDE5I treatment reduced the frequency of erections in BM-SS mice (1.7 ± 1.1 vs. 5.5 ± 2.8 erections per hour, P < 0.05). Penile tissues from BM-SS mice demonstrated decreased NOS, PKG, PDE5 and elevated ROS activities compared with that of control mice. PDE5I treatment increased NOS (11.6 ± 1.3% vs. 7.8 ± 2.3%, P < 0.05) and PDE5 (76.3 ± 9.8% vs. 52.3 ± 11.1%, P < 0.05) activities and decreased ROS activity (137.8 ± 12.1% vs. 199.1 ± 11.3%, P < 0.05) compared with non-PDE5I treated BM-SS mice. PKG activity was increased beyond control levels with PDE5I treatment (158.4 ± 10.3%, P < 0.05).

conclusionShort-term hemolysis is sufficient to establish a priapism phenotype and results in loss of erectile function. PDE5I treatment ameliorates priapism, in part, because of restored NO balance with decreased ROS generation and increased PDE5 activity.

Indexed as

HemolysisAcute DiseaseAnemia, Sickle CellAnimalsCyclic Nucleotide Phosphodiesterases, Type 5Disease Models, AnimalMaleMiceMice, TransgenicNitric Oxide SynthasePenile ErectionPenisPhosphodiesterase 5 InhibitorsPiperazinesPriapismSignal TransductionCyclic Nucleotide Phosphodiesterases, Type 5Nitric Oxide SynthasePde5a protein, mousePhosphodiesterase 5 InhibitorsPiperazinesSildenafil CitrateEndothelial Nitric Oxide SynthasePDE5 InhibitorPhosphodiesterase 5PriapismReactive Oxygen SpeciesSickle Cell Disease

Identifiers

PMID26346631
PMCPMC4600325
OpenAlexW2114348648

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.