ArticleJournal of neuroscience research2015
Early-stage development of human induced pluripotent stem cell-derived neurons.
Article in Journal of neuroscience research, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed, 23 citations in OpenAlex.
- Generation of Active Neurons from Mouse Embryonic Stem Cells Using Retinoic Acid and Purmorphamine.International journal of molecular sciences · 2025Article
- Impact of perfusion on neuronal development in human derived neuronal networks.APL bioengineering · 2024Article
- Review
- Non-uniform temporal scaling of developmental processes in the mammalian cortex.Nature communications · 2023Article
- Dendritic spine formation and synapse maturation in transcription factor-induced human iPSC-derived neurons.iScience · 2023Article
- Fluorescence-based actin turnover dynamics of stem cells as a profiling method for stem cell functional evolution, heterogeneity and phenotypic lineage parsing.Methods (San Diego, Calif.) · 2021Article
- Phosphorylation of GAP-43 T172 is a molecular marker of growing axons in a wide range of mammals including primates.Molecular brain · 2021Article
- Non-proliferative neurogenesis in human periodontal ligament stem cells.Scientific reports · 2019Article
- Characterization of Functional Primary Cilia in Human Induced Pluripotent Stem Cell-Derived Neurons.Neurochemical research · 2019Article
- Electrical Responses and Spontaneous Activity of Human iPS-Derived Neuronal Networks Characterized for 3-month Culture with 4096-Electrode Arrays.Frontiers in neuroscience · 2016Article
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Recent advances in human induced pluripotent stem cells (hiPSCs) offer new possibilities for biomedical research and clinical applications. Differentiated neurons from hiPSCs are expected to be useful for developing novel methods of treatment for various neurological diseases. However, the detailed process of functional maturation of hiPSC-derived neurons (hiPS neurons) remains poorly understood. This study analyzes development of hiPS neurons, focusing specifically on early developmental stages through 48 hr after cell seeding; development was compared with that of primary cultured neurons derived from the rat hippocampus. At 5 hr after cell seeding, neurite formation occurs in a similar manner in both neuronal populations. However, very few neurons with axonal polarization were observed in the hiPS neurons even after 48 hr, indicating that hiPS neurons differentiate more slowly than rat neurons. We further investigated the elongation speed of axons and found that hiPS neuronal axons were slower. In addition, we characterized the growth cones. The localization patterns of skeletal proteins F-actin, microtubule, and drebrin were similar to those of rat neurons, and actin depolymerization by cytochalasin D induced similar changes in cytoskeletal distribution in the growth cones between hiPS neurons and rat neurons. These results indicate that, during the very early developmental stage, hiPS neurons develop comparably to rat hippocampal neurons with regard to axonal differentiation, but the growth of axons is slower.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.