ArticleInternational journal of nanomedicine2015
A novel strategy for the discrimination of gelatinous Chinese medicines based on enzymatic digestion followed by nano-flow liquid chromatography in tandem with orbitrap mass spectrum detection.
Article in International journal of nanomedicine, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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12 citing papers in PubMed, 39 citations in OpenAlex.
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- Traditional Chinese medicine promotes bone regeneration in bone tissue engineering.Chinese medicine · 2022Review
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- Marker peptide screening and species-specific authentication of Pheretima using proteomics.Analytical and bioanalytical chemistry · 2021Article
- Collagen derived species-specific peptides for distinguishing donkey-hide gelatin (Chinese herbal medicines · 2021Article
- Specific, sensitive and rapid authentication of donkey-hide gelatine (Journal of food science and technology · 2020Article
- Glycyrrhizae Radix et Rhizoma Processed by Sulfur Fumigation Damaged the Chemical Profile Accompanied by Immunosuppression and Liver Injury.BioMed research international · 2020Article
- Harnessing multiplex PCR assay targeting specific mitochondrial DNA elements for simultaneous identification of antelope species inMitochondrial DNA. Part B, Resources · 2019Article
- Species-specific identification of collagen components in Colla corii asini using a nano-liquid chromatography tandem mass spectrometry proteomics approach.International journal of nanomedicine · 2017Article
- The identification of goat peroxiredoxin-5 and the evaluation and enhancement of its stability by nanoparticle formation.Scientific reports · 2016Article
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Authors and funding
7 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Gelatinous Chinese medicines made from mammalian skin or horn or reptile shell are a very important type of animal-derived Chinese medicine. They have been extensively used either as both hemopoietic and hemostatic agents to treat vertigo, palpitation, hematuria, and insomnia in traditional Chinese medicine clinics; consumed as a popular tonic for weaker persons such as the elderly or women after giving birth; or further manufactured to health supplements for certain populations. However, they cannot be discriminated from each other by only using the routine approach in the Chinese Pharmacopoeia, as it lacks enough specificity and, consequently, and the requirements can be met even by adding assayed ingredients. In this study, our efforts to differentiate three gelatinous Chinese medicines, Asini Corii Colla, Cervi Cornus Colla, and Testudinis Carapacis ET Plastri Colla, are presented, and a novel strategy based on enzymatic digestion followed by nano-flow liquid chromatography in tandem with orbitrap mass spectrum detector analysis is proposed herein. Fourteen diagnostic fragments identified from the digests of these medicines were exclusively selected for their discrimination. By taking advantage of the favorable features of this strategy, it is feasible and convenient to identify enzymatic-digested peptides originated from signature proteins in each medicine, which thus could be employed as potential biomarkers for their form of raw medicinal material, and the pulverized and the complex especially, that being the direct basis for authentication purpose.
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