ArticleScientific reports2015
Identification of residues important for substrate uptake in a glucose transporter from the filamentous fungus Trichoderma reesei.
Article in Scientific reports, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 53 citations in OpenAlex.
- Xy-31 and Lat-1 regulate the induction of polysaccharide disintegrating genes by arabinose inFrontiers in microbiology · 2026Article
- Advances in fungal sugar transporters: unlocking the potential of second-generation bioethanol production.Applied microbiology and biotechnology · 2025Review
- Transcriptomic and functional analyses on a Botrytis cinerea multidrug-resistant (MDR) strain provides new insights into the potential molecular mechanisms of MDR and fitness.Molecular plant pathology · 2024Article
- Performance evaluation of Trichoderma reseei in tolerance and biodegradation of diuron herbicide in agar plate, liquid culture and solid-state fermentation.World journal of microbiology & biotechnology · 2024Article
- Proteome profiling of enriched membrane-associated proteins unraveled a novel sophorose and cello-oligosaccharide transporter in Trichoderma reesei.Microbial cell factories · 2024Article
- Structural and biochemical insights of xylose MFS and SWEET transporters in microbial cell factories: challenges to lignocellulosic hydrolysates fermentation.Frontiers in microbiology · 2024Review
- An overview on current molecular tools for heterologous gene expression in Trichoderma.Fungal biology and biotechnology · 2021Review
- Functional characterization of a highly specific L-arabinose transporter from Trichoderma reesei.Microbial cell factories · 2021Article
- Electrophysiological characterization of a diverse group of sugar transporters from Trichoderma reesei.Scientific reports · 2021Article
- Article
- RCO-3 and COL-26 form an external-to-internal module that regulates the dual-affinity glucose transport system in Neurospora crassa.Biotechnology for biofuels · 2021Article
- The Plastidic Sugar Transporter pSuT Influences Flowering and Affects Cold Responses.Plant physiology · 2019Article
- CLP1, a Novel Plant Homeo Domain Protein, Participates in Regulating Cellulase Gene Expression in the Filamentous FungusFrontiers in microbiology · 2019Article
- New Genomic Approaches to Enhance Biomass Degradation by the Industrial FungusInternational journal of genomics · 2018Review
- The Promoter Toolbox for Recombinant Gene Expression inFrontiers in bioengineering and biotechnology · 2018Review
- Characterization of a novel sugar transporter involved in sugarcane bagasse degradation inBiotechnology for biofuels · 2018Article
- Characterization of the mammalian DEAD-box protein DDX5 reveals functional conservation withRNA (New York, N.Y.) · 2017Article
- The low affinity glucose transporter HxtB is also involved in glucose signalling and metabolism in Aspergillus nidulans.Scientific reports · 2017Article
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The glucose transporter is an important player in cell metabolism that mediates the intracellular uptake of glucose. Here, we characterized the glucose transporter Stp1 from the filamentous fungus Trichoderma reesei. The individual substitution of several conserved residues for Ala in Stp1 corresponding to those interacting with D-glucose in the xylose/H(+) symporter XylE inflicted contrasting effects on its ability to support the growth of an hxt-null yeast on glucose. The targeted change of Phe 50, proximal to the substrate-binding site, was also found to exert a profound effect on the activity of Stp1. In contrast with the charged residues, the substitution of Phe 50 with either the hydrophilic residues Asn and Gln or the small residues Gly and Ala significantly enhanced the transport of glucose and its fluorescent analogue, 2-NBDG. On the other hand, a variant with the three substitutions I115F, F199I and P214L displayed remarkably improved activity on glucose and 2-NBDG transport. Further analysis indicated that the combined mutations of Ile 115 and Pro 214, positioned on the lateral surface of the Stp1 N-domain, fully accounted for the enhanced transport activity. These results provide insight into the structural basis for glucose uptake in fungal sugar transporters.
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