ArticleNature communications2015
Modular pathway rewiring of Saccharomyces cerevisiae enables high-level production of L-ornithine.
Article in Nature communications, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 45 papers.
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Who cites it
45 citing papers in PubMed.
- System reconstruction ofSynthetic and systems biotechnology · 2027Article
- Metabolic and Synthetic Biology Strategies for Enhancing Single-Cell Protein Production in Saccharomyces cerevisiae.Biotechnology journal · 2026Review
- Synthesis of Amines for Active Pharmaceutical Ingredients Using the Whole-Cell Factory Saccharomyces Cerevisae.Chembiochem : a European journal of chemical biology · 2026Review
- Automatic Blood Protein Enrichment by Magnetic-COF Polymers.Analytical chemistry · 2026Article
- Isolation and characterization of Saccharomyces cerevisiae mutants with ornithine accumulation for value-added craft beer brewing.Journal of industrial microbiology & biotechnology · 2026Article
- Comparing metabolic engineering scenarios using simulated design-build-test-learn-cycles.Frontiers in bioengineering and biotechnology · 2026Article
- Efficient fermentative production of SAM through multidimensional engineering of Saccharomyces cerevisiae.World journal of microbiology & biotechnology · 2025Article
- Enhancing cellulase biosynthesis ofiScience · 2025Article
- Modular deregulation of central carbon metabolism for efficient xylose utilization in Saccharomyces cerevisiae.Nature communications · 2025Article
- Comprehensive evaluation of the capacities of microbial cell factories.Nature communications · 2025Article
- Overexpression of arginase geneSynthetic and systems biotechnology · 2024Article
- A promoter-RBS library for fine-tuning gene expression inApplied and environmental microbiology · 2024Article
- Multivariate modular metabolic engineering and medium optimization for vitamin BSynthetic and systems biotechnology · 2024Article
- A novel strategy for L-arginine production in engineered Escherichia coli.Microbial cell factories · 2023Article
- Engineering yeast mitochondrial metabolism for 3-hydroxypropionate production.Biotechnology for biofuels and bioproducts · 2023Article
- Metabolic Engineering of Microorganisms to Produce Pyruvate and Derived Compounds.Molecules (Basel, Switzerland) · 2023Review
- Comprehensive Analysis of Signal Peptides in Saccharomyces cerevisiae Reveals Features for Efficient Secretion.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2023Article
- Development of an industrial yeast strain for efficient production of 2,3-butanediol.Microbial cell factories · 2022Article
- Enhanced L-ornithine production from glucose and sucrose via manipulation of the fructose metabolic pathway in Corynebacterium glutamicum.Bioresources and bioprocessing · 2022Article
- Toward improved terpenoids biosynthesis: strategies to enhance the capabilities of cell factories.Bioresources and bioprocessing · 2022Review
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Authors and funding
9 authors.
Funding
Abstract
Baker's yeast Saccharomyces cerevisiae is an attractive cell factory for production of chemicals and biofuels. Many different products have been produced in this cell factory by reconstruction of heterologous biosynthetic pathways; however, endogenous metabolism by itself involves many metabolites of industrial interest, and de-regulation of endogenous pathways to ensure efficient carbon channelling to such metabolites is therefore of high interest. Furthermore, many of these may serve as precursors for the biosynthesis of complex natural products, and hence strains overproducing certain pathway intermediates can serve as platform cell factories for production of such products. Here we implement a modular pathway rewiring (MPR) strategy and demonstrate its use for pathway optimization resulting in high-level production of L-ornithine, an intermediate of L-arginine biosynthesis and a precursor metabolite for a range of different natural products. The MPR strategy involves rewiring of the urea cycle, subcellular trafficking engineering and pathway re-localization, and improving precursor supply either through attenuation of the Crabtree effect or through the use of controlled fed-batch fermentations, leading to an L-ornithine titre of 1,041±47 mg l(-1) with a yield of 67 mg (g glucose)(-1) in shake-flask cultures and a titre of 5.1 g l(-1) in fed-batch cultivations. Our study represents the first comprehensive study on overproducing an amino-acid intermediate in yeast, and our results demonstrate the potential to use yeast more extensively for low-cost production of many high-value amino-acid-derived chemicals.
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