Evidence map›Paper›PMID 26344899›Full record

ArticleProtein expression and purification2016

Over-expression, purification, and confirmation of Bacillus anthracis transcriptional regulator NprR.

Amy J Rice, Jerry K Woo, Attiya Khan, Michael Z Szypulinski, Michael E Johnson, Hyunwoo Lee, Hyun Lee

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Protein expression and purification, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.2field-weighted citation impact, top 44% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 5 citations in OpenAlex.

  1. Article
  2. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 1 institution in 1 country.

Amy J RiceCenter for Pharmaceutical Biotechnology, University of Illinois at Chicago, 900 S. Ashland, IL 60607, USA; Department of Medicinal Chemistry and Pharmacognosy, University of Illinois at Chicago, 900 S. Ashland, IL 60607, USA.
Jerry K WooCenter for Pharmaceutical Biotechnology, University of Illinois at Chicago, 900 S. Ashland, IL 60607, USA.
Attiya KhanCenter for Pharmaceutical Biotechnology, University of Illinois at Chicago, 900 S. Ashland, IL 60607, USA.
Michael Z SzypulinskiCenter for Pharmaceutical Biotechnology, University of Illinois at Chicago, 900 S. Ashland, IL 60607, USA.
Michael E JohnsonCenter for Pharmaceutical Biotechnology, University of Illinois at Chicago, 900 S. Ashland, IL 60607, USA; Department of Medicinal Chemistry and Pharmacognosy, University of Illinois at Chicago, 900 S. Ashland, IL 60607, USA.
Hyunwoo LeeCenter for Pharmaceutical Biotechnology, University of Illinois at Chicago, 900 S. Ashland, IL 60607, USA; Department of Biopharmaceutical Sciences, University of Illinois at Chicago, 900 S. Ashland, IL 60607, USA. Electronic address: hlee31@uic.edu.
Hyun LeeCenter for Pharmaceutical Biotechnology, University of Illinois at Chicago, 900 S. Ashland, IL 60607, USA; Department of Medicinal Chemistry and Pharmacognosy, University of Illinois at Chicago, 900 S. Ashland, IL 60607, USA. Electronic address: danielhl@uic.edu.
University of Illinois Chicago · US

Funding

Multidisciplinary Oral Science TrainingT32DE018381 · NIDCR · UNIVERSITY OF ILLINOIS AT CHICAGO · PI COOPER, LYNDON F · 2008 to 2019
$3.6M
Peptide-Based Quorum Sensing Controlling Virulence in Bacillus anthracisR01AI095488 · NIAID · UNIVERSITY OF ILLINOIS AT CHICAGO · PI LEE, HYUNWOO · 2012 to 2015
$1.6M
NIAID NIH HHS R01 AI095488NIDCR NIH HHS T32 DE018381
6 · The paper itself

Abstract

Quorum sensing (QS) has been recognized as an important biological phenomenon in which bacterial cells communicate and coordinate their gene expression and cellular processes with respect to population density. Bacillus anthracis is the etiological agent of fatal pulmonary anthrax infections, and the NprR/NprX QS system may be involved in its pathogenesis. NprR, renamed as aqsR for anthrax quorum sensing Regulator, is a transcriptional regulator that may control the expression of genes required for proliferation and survival. Currently, there is no protocol reported to over-express and purify B. anthracis AqsR. In this study, we describe cloning, purification, and confirmation of functional full-length B. anthracis AqsR protein. The AqsR gene was cloned into the pQE-30 vector with an HRV 3C protease recognition site between AqsR and the N-terminal His6-tag in order to yield near native AqsR after the His-tag cleavage, leaving only two additional amino acid residues at the N-terminus.

Indexed as

Bacterial ProteinsGene Expression Regulation, BacterialTranscription FactorsBacillus anthracisCell LineGene ExpressionHumansQuorum SensingSurface Plasmon ResonanceTandem Mass SpectrometryBacterial ProteinsTranscription FactorsBacillus anthracisPurificationQuorum sensingThe neutral protease regulator (NprR)

Identifiers

PMID26344899
PMCPMC4853309
OpenAlexW1793283016

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.