Evidence map›Paper›PMID 26344757›Full record

ArticleMethods (San Diego, Calif.)2016

Micropost arrays for measuring stem cell-derived cardiomyocyte contractility.

Kevin M Beussman, Marita L Rodriguez, Andrea Leonard, Nikita Taparia, Curtis R Thompson, Nathan J Sniadecki

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Methods (San Diego, Calif.), 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 46 papers.

0numbers the graph read from it
0cells of the map it votes in
46citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

46 citing papers in PubMed.

  1. Article
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  3. Article
  4. Review
  5. Review
  6. Simultaneous Measurement of Contractile Force and CaSensors (Basel, Switzerland) · 2025
    Article
  7. Review
  8. Review
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  11. Article
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  13. Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Kevin M BeussmanDepartment of Mechanical Engineering, University of Washington, Seattle, WA, USA.
Marita L RodriguezDepartment of Mechanical Engineering, University of Washington, Seattle, WA, USA.
Andrea LeonardDepartment of Mechanical Engineering, University of Washington, Seattle, WA, USA.
Nikita TapariaDepartment of Mechanical Engineering, University of Washington, Seattle, WA, USA.
Curtis R ThompsonDepartment of Mechanical Engineering, University of Washington, Seattle, WA, USA.
Nathan J SniadeckiDepartment of Mechanical Engineering, University of Washington, Seattle, WA, USA; Department of Bioengineering, University of Washington, Seattle, WA, USA. Electronic address: nsniadec@uw.edu.

Funding

Bioengineering Cardiovascular Training GrantT32EB001650 · NIBIB · UNIVERSITY OF WASHINGTON · PI REGNIER, MICHAEL · 2004 to 2019
$3.6M
Integrins, mechanotransduction, and the maturation of cardiomyocytes derived from induced pluripotent stem cellsF32HL126332 · NHLBI · UNIVERSITY OF WASHINGTON · PI LEONARD, ANDREA B · 2015 to 2017
$164k
NHLBI NIH HHS F32 HL126332NHLBI NIH HHS HL126332NIBIB NIH HHS T32 EB001650
6 · The paper itself

Abstract

Stem cell-derived cardiomyocytes have the potential to be used to study heart disease and maturation, screen drug treatments, and restore heart function. Here, we discuss the procedures involved in using micropost arrays to measure the contractile forces generated by stem cell-derived cardiomyocytes. Cardiomyocyte contractility is needed for the heart to pump blood, so measuring the contractile forces of cardiomyocytes is a straightforward way to assess their function. Microfabrication and soft lithography techniques are utilized to create identical arrays of flexible, silicone microposts from a common master. Micropost arrays are functionalized with extracellular matrix protein to allow cardiomyocytes to adhere to the tips of the microposts. Live imaging is used to capture videos of the deflection of microposts caused by the contraction of the cardiomyocytes. Image analysis code provides an accurate means to quantify these deflections. The contractile forces produced by a beating cardiomyocyte are calculated by modeling the microposts as cantilever beams. We have used this assay to assess techniques for improving the maturation and contractile function of stem cell-derived cardiomyocytes.

Indexed as

Cell AdhesionCells, CulturedHumansInduced Pluripotent Stem CellsMyocardial ContractionMyocytes, CardiacSingle-Cell AnalysisCardiomyocytesCell mechanicsInduced pluripotent stem cellsMicropostsSoft lithography

Identifiers

PMID26344757
PMCPMC4761463

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.