Evidence map›Paper›PMID 26344226›Full record

ArticleCurrent protocols in nucleic acid chemistry2015

Separation of Quadruplex Polymorphism in DNA Sequences by Reversed-Phase Chromatography.

M Clarke Miller, Carl J Ohrenberg, Ashani Kuttan, John O Trent

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Current protocols in nucleic acid chemistry, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. G-Quadruplex Secondary Structure Obtained from Circular Dichroism Spectroscopy.Angewandte Chemie (International ed. in English) · 2018
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

M Clarke MillerDepartment of Chemistry and Biochemistry, University of North Georgia, Oakwood, Georgia.
Carl J OhrenbergDepartment of Chemistry and Biochemistry, University of North Georgia, Oakwood, Georgia.
Ashani KuttanJames Graham Brown Cancer Center, University of Louisville, Louisville, Kentucky.
John O TrentJames Graham Brown Cancer Center, University of Louisville, Louisville, Kentucky.

Funding

PILOT PROJECT PROGRAMP30GM106396 · NIGMS · UNIVERSITY OF LOUISVILLE · PI EATON, JOHN W · 2013 to 2017
$5.4M
Targeting Nucleic Acids with an Integrated Vitural and Actual ScreenR01GM077422 · NIGMS · UNIVERSITY OF LOUISVILLE · PI CHAIRES, JONATHAN B., TRENT, JOHN O · 2007 to 2020
$3.7M
NIGMS NIH HHS GM077422NIGMS NIH HHS P30 GM106396NIGMS NIH HHS R01 GM077422
6 · The paper itself

Abstract

This unit describes a method for the separation of a mixture of quadruplex conformations formed from the same parent sequence via reversed-phase chromatography (RPC). Polymorphism is inherent to quadruplex formation and even relatively simple quadruplex-forming sequences can fold into a cornucopia of possible conformations and topologies. Isolation of a specific conformation for study can be problematic. This is especially true for conformations of the human telomere sequence d(GGG(TTAGGG)3). High performance liquid chromatography (HPLC), especially reversed-phase chromatography, has been a mainstay of nucleic acid research and purification for many decades. We have successfully applied this method to the problem of separating individual quadruplex species in the ensemble from the same parent sequence.

Indexed as

G-QuadruplexesChromatography, Reverse-PhaseDNAHumansPolymerase Chain ReactionTelomereDNAquadruplexreversed-phase chromatographyRPCtelomere

Identifiers

PMID26344226
PMCPMC4561857

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.