ArticleThe Laryngoscope2016
Vocal fold myofibroblast profile of scarring.
Article in The Laryngoscope, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 34 citations in OpenAlex.
- Controlled decorin delivery from injectable microgels promotes scarless vocal fold repair.bioRxiv : the preprint server for biology · 2025Article
- Nanoparticle-mediated targeting of PGC-1α reveals critical metabolic pathways in bladder cancer metastasis.Communications biology · 2025Article
- Inhibition of the MRTF-A/SRF signaling axis alleviates vocal fold scarring.Matrix biology : journal of the International Society for Matrix Biology · 2025Article
- RNA-sequencing demonstrates transcriptional differences between human vocal fold fibroblasts and myofibroblasts.BMC genomics · 2025Article
- Biophysical aspects of mechanotransduction in cells and their physiological/biological implications in vocal fold vibration: a narrative review.Frontiers in cell and developmental biology · 2025Review
- Concentration Effects of Methylprednisolone in Human Vocal Fold Fibroblast-Macrophage Co-Culture.The Laryngoscope · 2023Article
- Dose-Dependent Glucocorticoid Regulation of Transcription Factors in Vocal Fold Fibroblasts and Macrophages.The Laryngoscope · 2023Article
- A roadmap for developing and engineeringBiophysics reviews · 2023Review
- Larynx proteomics after jellyfish collagen IL: Increased ECM/collagen and suppressed inflammation.Laryngoscope investigative otolaryngology · 2022Article
- Bioreactors for Vocal Fold Tissue Engineering.Tissue engineering. Part B, Reviews · 2022Review
- Progress in Vocal Fold Regenerative Biomaterials: An Immunological Perspective.Advanced nanobiomed research · 2022Article
- Antifibrotic effects of Quercetin on TGF-β1-induced vocal fold fibroblasts.American journal of translational research · 2022Article
- Concurrent YAP/TAZ and SMAD signaling mediate vocal fold fibrosis.Scientific reports · 2021Article
- Regulation of Stem Cell Function in an Engineered Vocal Fold-Mimetic Environment.Regenerative engineering and translational medicine · 2020Article
- Potential effect of non-thermal plasma for the inhibition of scar formation: a preliminary report.Scientific reports · 2020Article
- In vitro mechanical vibration down-regulates pro-inflammatory and pro-fibrotic signaling in human vocal fold fibroblasts.PloS one · 2020Article
- Reversal of Vocal Fold Mucosal Fibrosis Using siRNA against the Collagen-Specific Chaperone Serpinh1.Molecular therapy. Nucleic acids · 2019Article
- Pathophysiology of Fibrosis in the Vocal Fold: Current Research, Future Treatment Strategies, and Obstacles to Restoring Vocal Fold Pliability.International journal of molecular sciences · 2019Review
- In vitro evaluation of anti-fibrotic effects of select cytokines for vocal fold scar treatment.Journal of biomedical materials research. Part B, Applied biomaterials · 2019Article
- Tissue specific human fibroblast differential expression based on RNAsequencing analysis.BMC genomics · 2019Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
Abstract
objectives/hypothesisVocal fold fibroblasts (VFF) are responsible for extracellular matrix synthesis supporting lamina propria in normal and diseased conditions. When tissue is injured, VFF become activated and differentiate into myofibroblasts to facilitate wound healing response. We investigated if vocal fold myofibroblasts can be utilized as surrogate cells for scarred VFF. STUDY
designIn vitro.
methodsNormal VFF cell lines from a 21-year-old male (N21), 59-year-old female (N59), and a scar VFF cell line from a 56-year-old female (S56) were used in this study. 10 ng/mL of transforming growth factor (TGFβ1) was applied for 5 days to normal VFF. Myofibroblast differentiation was determined with immunocytochemistry and western blot, measuring alpha smooth muscle actin (α-SMA). Cell growth, proliferation, contractile properties, and gene expression profiles were evaluated.
resultsN21, N59, and S56 VFF presented elongated configuration. N21+ and N21- VFF demonstrated significantly greater proliferation compared to N59+, N59-, and S56 VFF at 6 days. α-SMA was expressed in all cells. Fibronectin, alpha smooth actin, connective tissue growth factor, and metallopeptidase inhibitor were the highest genes expression in VFF treated with transforming growth factor β1 (TGFβ1). At 24 hours, S56 VFF showed lower contraction compared to N21+ and N59+ VFF, but at 60 hours S56 VFF had lower collagen contraction compared to all cell groups. Highest collagen contraction matrices were measured with VFF treated with TGFβ1 at 24 hours and N59- VFF at 60 hours.
conclusionVFF treated with TGFβ1 (myofibroblasts) appear to have similar phenotypic characteristics but different genotypic behavior compared to scar VFF. LEVEL OF EVIDENCE: N/A. Laryngoscope, 126:E110-E117, 2016.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.