ArticleInternational journal of molecular sciences2015
Evaluation of Appropriate Reference Genes for Reverse Transcription-Quantitative PCR Studies in Different Tissues of a Desert Poplar via Comparision of Different Algorithms.
Article in International journal of molecular sciences, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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21 citing papers in PubMed, 40 citations in OpenAlex.
- Article
- Genome-Wide Identification and Functional Analysis ofInternational journal of molecular sciences · 2025Article
- Comprehensive Genomic Analysis of Trihelix Transcription Factor Genes and Their Expression Underlying Abiotic Stress in Euphrates Poplar (Plants (Basel, Switzerland) · 2025Article
- A Genome-Wide Characterization of Receptor-like Cytoplasmic Kinase IV Subfamily Members inPlants (Basel, Switzerland) · 2024Article
- Identification of the Optimal Quantitative RT-PCR Reference Gene for Paper Mulberry (Current issues in molecular biology · 2024Article
- Transcriptome-Based Identification of the Optimal Reference Genes for Quantitative Real-Time Polymerase Chain Reaction Analyses of Lingonberry Fruits throughout the Growth Cycle.Plants (Basel, Switzerland) · 2023Article
- Aspartyl tRNA-synthetase (AspRS) gene family enhances drought tolerance in poplar through BABA-PtrIBIs-PtrVOZ signaling module.BMC genomics · 2023Article
- Genome-Wide Analysis of the FBA Subfamily of the Poplar F-Box Gene Family and Its Role under Drought Stress.International journal of molecular sciences · 2023Article
- Transcriptome and metabolome analyses reveal molecular mechanisms of anthocyanin-related leaf color variation in poplar (Frontiers in plant science · 2023Article
- Identification of suitable reference genes for quantitative reverse transcription PCR inPhysiology and molecular biology of plants : an international journal of functional plant biology · 2022Article
- Genome-Wide Comprehensive Analysis of theInternational journal of molecular sciences · 2021Article
- The Receptor-Like KinaseInternational journal of molecular sciences · 2021Article
- Article
- Poplar Autophagy Receptor NBR1 Enhances Salt Stress Tolerance by Regulating Selective Autophagy and Antioxidant System.Frontiers in plant science · 2020Article
- Conjoint Analysis of Genome-Wide lncRNA and mRNA Expression of Heteromorphic Leavesin Response to Environmental HeterogeneityinInternational journal of molecular sciences · 2019Article
- Selection of Appropriate Reference Genes for Gene Expression Analysis under Abiotic Stresses inInternational journal of molecular sciences · 2019Article
- Comparison of Reliable Reference Genes Following Different Hormone Treatments by Various Algorithms for qRT-PCR Analysis ofInternational journal of molecular sciences · 2018Article
- Article
- A simple method to assess group difference in RT-qPCR reference gene selection using GeNorm: The case of the placental sex.Scientific reports · 2017Article
- Selection of Suitable Reference Genes for Quantitative Real-time PCR inFrontiers in plant science · 2017Article
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Authors and funding
11 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Despite the unshakable status of reverse transcription-quantitative PCR in gene expression analysis, it has certain disadvantages, including that the results are highly dependent on the reference genes selected for data normalization. Since inappropriate endogenous control genes will lead to inaccurate target gene expression profiles, the validation of suitable internal reference genes is essential. Given the increasing interest in functional genes and genomics of Populus euphratica, a desert poplar showing extraordinary adaptation to salt stress, we evaluated the expression stability of ten candidate reference genes in P. euphratica roots, stems, and leaves under salt stress conditions. We used five algorithms, namely, ΔCt, NormFinder, geNorm, GrayNorm, and a rank aggregation method (RankAggreg) to identify suitable normalizers. To support the suitability of the identified reference genes and to compare the relative merits of these different algorithms, we analyzed and compared the relative expression levels of nine P. euphratica functional genes in different tissues. Our results indicate that a combination of multiple reference genes recommended by GrayNorm algorithm (e.g., a combination of Actin, EF1α, GAPDH, RP, UBQ in root) should be used instead of a single reference gene. These results are valuable for research of gene identification in different P. euphratica tissues.
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