ArticleMolecules (Basel, Switzerland)2015
Optimization of Ultrasonic-Assisted Extraction of Flavonoid Compounds and Antioxidants from Alfalfa Using Response Surface Method.
Article in Molecules (Basel, Switzerland), 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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27 citing papers in PubMed.
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- Pharmacological Activities ofMolecules (Basel, Switzerland) · 2024Review
- Ultrasound assisted phytochemical extraction of red cabbage by using deep eutectic solvent: Modelling using ANFIS and optimization by genetic algorithms.Ultrasonics sonochemistry · 2024Article
- Total Flavonoids inInternational journal of molecular sciences · 2023Article
- Study of Influence of Extraction Method on the Recovery Bioactive Compounds from Peel Avocado.Molecules (Basel, Switzerland) · 2023Article
- Hydrolipidic Characteristics and Clinical Efficacy of a Dermocosmetic Formulation for the Improvement of Homeostasis on Oily Mature Skin.Life (Basel, Switzerland) · 2022Article
- Effects of Extraction Technique on the Content and Antioxidant Activity of Flavonoids from GossypiumMolecules (Basel, Switzerland) · 2022Article
- Extraction of Lipophilic Antioxidants from Native Tomato Using Green Technologies.Food technology and biotechnology · 2022Article
- Multi-omics analyses reveal new insights into nutritional quality changes of alfalfa leaves during the flowering period.Frontiers in plant science · 2022Article
- Procyanidins: From Agro-Industrial Waste to Food as Bioactive Molecules.Foods (Basel, Switzerland) · 2021Review
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- Nutritional, Antioxidant, Antimicrobial, and Toxicological Profile of Two Innovative Types of Vegan, Sugar-Free Chocolate.Foods (Basel, Switzerland) · 2020Article
- A green extraction design for enhancing flavonoid compounds fromRoyal Society open science · 2020Article
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3 authors.
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Abstract
Ultrasonic-assisted extraction (UAE) was used to extract flavonoid-enriched antioxidants from alfalfa aerial part. Response surface methodology (RSM), based on a four-factor, five-level central composite design (CCD), was employed to obtain the optimal extraction parameters, in which the flavonoid content was maximum and the antioxidant activity of the extracts was strongest. Radical scavenging capacity of the extracts, which represents the amounts of antioxidants in alfalfa, was determined by using 2,2'-azino-bis (3-ethylbenzothiazoline-6-sulphonicacid) (ABTS) and 2,2'-diphenyl-1-picrylhydrazyl (DPPH) methods. The results showed good fit with the proposed models for the total flavonoid extraction (R² = 0.9849), for the antioxidant extraction assayed by ABTS method (R² = 0.9764), and by DPPH method (R² = 0.9806). Optimized extraction conditions for total flavonoids was a ratio of liquid to solid of 57.16 mL/g, 62.33 °C, 57.08 min, and 52.14% ethanol. The optimal extraction parameters of extracts for the highest antioxidant activity by DPPH method was a ratio of liquid to solid 60.3 mL/g, 54.56 °C, 45.59 min, and 46.67% ethanol, and by ABTS assay was a ratio of liquid to solid 47.29 mL/g, 63.73 °C, 51.62 min, and 60% ethanol concentration. Our work offers optimal extraction conditions for total flavonoids and antioxidants from alfalfa.
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