Evidence map›Paper›PMID 26342906›Full record

ArticleJournal of virological methods2015

A novel diagnostic approach to detecting porcine epidemic diarrhea virus: The lateral immunochromatography assay.

Yong Kwan Kim, Seong-In Lim, In-Soo Cho, Kwang-Myun Cheong, Eun-Jeong Lee, Sang-Oh Lee, Joon-Bae Kim, Jung-Hwa Kim, Dong-Soo Jeong, Byung-Hyun An and 1 more

Open access · greenAbstract readComparative StudyEvaluation Study
In one paragraph

Article in Journal of virological methods, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
18citing papers in PubMed, 1 pooled it
3.3field-weighted citation impact, top 10% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

18 citing papers in PubMed, 1 synthesis or guideline pooled it, 24 citations in OpenAlex.

  1. Pooled it
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  7. Establishment of method for dual simultaneous detection of PEDV and TGEV by combination of magnetic micro-particles and nanoparticles.Journal of infection and chemotherapy : official journal of the Japan Society of Chemotherapy · 2020
    Article
  8. Article
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  15. Article
  16. Lateral flow assays.Essays in biochemistry · 2016
    Review
  17. Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors at 2 institutions in 1 country.

Yong Kwan KimViral Disease Division, Animal and Plant Quarantine Agency, Anyang, Gyeonggi-do 430-757, Republic of Korea.
Seong-In LimViral Disease Division, Animal and Plant Quarantine Agency, Anyang, Gyeonggi-do 430-757, Republic of Korea.
In-Soo ChoViral Disease Division, Animal and Plant Quarantine Agency, Anyang, Gyeonggi-do 430-757, Republic of Korea.
Kwang-Myun CheongMedian Diagnostics Inc., Chuncheon, Gangwon-do 200-883, Republic of Korea.
Eun-Jeong LeeMedian Diagnostics Inc., Chuncheon, Gangwon-do 200-883, Republic of Korea.
Sang-Oh LeeMedian Diagnostics Inc., Chuncheon, Gangwon-do 200-883, Republic of Korea.
Joon-Bae KimMedian Diagnostics Inc., Chuncheon, Gangwon-do 200-883, Republic of Korea.
Jung-Hwa KimMedian Diagnostics Inc., Chuncheon, Gangwon-do 200-883, Republic of Korea.
Dong-Soo JeongGangwon-do Veterinary Service Laboratory, Chuncheon, Gangwon-do 200-822, Republic of Korea.
Byung-Hyun AnApplied Chemistry and Biological Engineering, Ajou University, Suwon 443-749, Republic of Korea.
Dong-Jun AnViral Disease Division, Animal and Plant Quarantine Agency, Anyang, Gyeonggi-do 430-757, Republic of Korea. Electronic address: andj67@korea.kr.
Animal and Plant Quarantine Agency · KRAjou University · KR

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Porcine epidemic diarrhea virus (PEDV) causes acute diarrhea and dehydration in sucking piglets and has a high mortality rate. An immunochromatography (IC) assay, known as a lateral flow test, is a simple device intended to detect the presence of target pathogens. Here, we developed an IC assay that detected PEDV antigens with 96.0% (218/227) sensitivity and 98.5% (262/266) specificity when compared with real-time reverse transcriptase (RT)-PCR using FAM-labeled probes based on sequences from nucleocapsid genes. The detection limits of the real-time RT-PCR and IC assays were 1×10(2) and 1×10(3) copies, respectively. The IC assay developed herein did not detect non-specific reactions with other viral or bacterial pathogens, and the assay could be stored at 4°C or room temperature for 15 months without affecting its efficacy. Thus, the IC assay may result in improved PED detection and control on farms, and is a viable alternative to current diagnostic tools for PEDV.

Indexed as

AnimalsAntigens, ViralChromatography, AffinityCoronavirus InfectionsPorcine epidemic diarrhea virusReal-Time Polymerase Chain ReactionReverse Transcriptase Polymerase Chain ReactionSensitivity and SpecificitySwineSwine DiseasesAntigens, ViralImmunochromatographyLateral flow testPorcine epidemic diarrhea virusSensitivitySpecificity

Identifiers

PMID26342906
PMCPMC7119843
OpenAlexW1212923862

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.