ArticleThe Journal of biological chemistry2015
Sph3 Is a Glycoside Hydrolase Required for the Biosynthesis of Galactosaminogalactan in Aspergillus fumigatus.
Article in The Journal of biological chemistry, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 51 papers.
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Who cites it
51 citing papers in PubMed, 99 citations in OpenAlex.
- Bacterial bioactive metabolites can facilitate cooperative interactions withinApplied and environmental microbiology · 2026Article
- Galactosaminogalactan orchestrates Verticillium dahliae virulence and rhizosphere microbial ecology through multi-partite interactions.The ISME journal · 2026Article
- MaMsb2, a signaling mucin, is involved in conidiation, stress tolerances, and virulence in the entomopathogenic fungusVirulence · 2025Article
- TheJournal of fungi (Basel, Switzerland) · 2025Article
- Regulation of Necroptosis by Aspergillus fumigatus Exopolysaccharide in Human Macrophages Derived from THP-1 Cells: Involvement of the RIP1-RIP3-MLKL Signaling Axis.Current microbiology · 2025Article
- Structures of α-galactosaminidases from the CAZy GH114 family and homologs defining a new GH191 family of glycosidases.Acta crystallographica. Section D, Structural biology · 2025Article
- Genomic Insights into Cobweb Disease Resistance inJournal of fungi (Basel, Switzerland) · 2025Article
- Characterization of theMicroorganisms · 2024Article
- CD56-mediated activation of human natural killer cells is triggered by Aspergillus fumigatus galactosaminogalactan.PLoS pathogens · 2024Article
- The Response of Growth and Transcriptome Profiles of Tea Grey Blight Disease PathogenInternational journal of molecular sciences · 2024Article
- Secretome Analysis ofJournal of fungi (Basel, Switzerland) · 2024Article
- Universal microbial reworking of dissolved organic matter along environmental gradients.Nature communications · 2024Article
- UnderstandingCell surface (Amsterdam, Netherlands) · 2023Review
- Genomics of soil depth niche partitioning in the Thaumarchaeota family Gagatemarchaeaceae.Nature communications · 2023Article
- Pseudomonas aeruginosa biofilm exopolysaccharides: assembly, function, and degradation.FEMS microbiology reviews · 2023Review
- Strategy to combat biofilms: a focus on biofilm dispersal enzymes.NPJ biofilms and microbiomes · 2023Review
- Identification of Compounds PreventingInternational journal of molecular sciences · 2023Article
- Article
- Aspergillus fumigatus Elongator complex subunit 3 affects hyphal growth, adhesion and virulence through wobble uridine tRNA modification.PLoS pathogens · 2022Article
- Filamentous fungal biofilms: Conserved and unique aspects of extracellular matrix composition, mechanisms of drug resistance and regulatory networks in Aspergillus fumigatus.NPJ biofilms and microbiomes · 2022Review
Corrections and comments
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Authors and funding
13 authors at 4 institutions in 2 countries.
Funding
Abstract
Aspergillus fumigatus is the most virulent species within the Aspergillus genus and causes invasive infections with high mortality rates. The exopolysaccharide galactosaminogalactan (GAG) contributes to the virulence of A. fumigatus. A co-regulated five-gene cluster has been identified and proposed to encode the proteins required for GAG biosynthesis. One of these genes, sph3, is predicted to encode a protein belonging to the spherulin 4 family, a protein family with no known function. Construction of an sph3-deficient mutant demonstrated that the gene is necessary for GAG production. To determine the role of Sph3 in GAG biosynthesis, we determined the structure of Aspergillus clavatus Sph3 to 1.25 Å. The structure revealed a (β/α)8 fold, with similarities to glycoside hydrolase families 18, 27, and 84. Recombinant Sph3 displayed hydrolytic activity against both purified and cell wall-associated GAG. Structural and sequence alignments identified three conserved acidic residues, Asp-166, Glu-167, and Glu-222, that are located within the putative active site groove. In vitro and in vivo mutagenesis analysis demonstrated that all three residues are important for activity. Variants of Asp-166 yielded the greatest decrease in activity suggesting a role in catalysis. This work shows that Sph3 is a glycoside hydrolase essential for GAG production and defines a new glycoside hydrolase family, GH135.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.