ArticleThe Journal of biological chemistry2015
Two Conserved Cysteine Residues Are Required for the Masculinizing Activity of the Silkworm Masc Protein.
Article in The Journal of biological chemistry, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed.
- Molecular mechanisms of sex determination in Lepidoptera: current status and perspectives.Insect science · 2026Review
- The Masc-PSI complex directly induces male-type doublesex splicing in silkworms.Communications biology · 2025Article
- Zygosity-based sex determination in a butterfly drives hypervariability ofScience advances · 2024Article
- Silencing RNAs expressed from W-linkedProceedings of the National Academy of Sciences of the United States of America · 2022Article
- A Wolbachia factor for male killing in lepidopteran insects.Nature communications · 2022Article
- CRISPR/Cas9-Mediated Mutagenesis of Sex-SpecificCells · 2022Article
- Article
- Identification and Characterization of the Masculinizing Function of theInternational journal of molecular sciences · 2021Article
- A conserved role of the duplicated Masculinizer gene in sex determination of the Mediterranean flour moth, Ephestia kuehniella.PLoS genetics · 2021Article
- The Sex Determination Cascade in the Silkworm.Genes · 2021Review
- Transgenic and knockout analyses of Masculinizer and doublesex illuminated the unique functions of doublesex in germ cell sexual development of the silkworm, Bombyx mori.BMC developmental biology · 2020Article
- Spliceosomal Protein GeneInternational journal of molecular sciences · 2020Article
- Identification and characterization of a Masculinizer homologue in the diamondback moth, Plutella xylostella.Insect molecular biology · 2020Article
- Masc-induced dosage compensation in silkworm cultured cells.FEBS open bio · 2019Article
- A Novel Splice Variant of the Masculinizing GeneBiomolecules · 2019Article
- Alternative splicing regulation of doublesex gene by RNA-binding proteins in the silkworm Bombyx mori.RNA biology · 2019Article
- Bombyx mori histone methyltransferase BmAsh2 is essential for silkworm piRNA-mediated sex determination.PLoS genetics · 2018Article
- The genome of the Hi5 germ cell line fromeLife · 2018Article
- Unique sex determination system in the silkworm, Bombyx mori: current status and beyond.Proceedings of the Japan Academy. Series B, Physical and biological sciences · 2018Review
- Transgenic Expression of the piRNA-Resistant Masculinizer Gene Induces Female-Specific Lethality and Partial Female-to-Male Sex Reversal in the Silkworm, Bombyx mori.PLoS genetics · 2016Article
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4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We have recently discovered that the Masculinizer (Masc) gene encodes a CCCH tandem zinc finger protein, which controls both masculinization and dosage compensation in the silkworm Bombyx mori. In this study, we attempted to identify functional regions or residues that are required for the masculinizing activity of the Masc protein. We constructed a series of plasmids that expressed the Masc derivatives and transfected them into a B. mori ovary-derived cell line, BmN-4. To assess the masculinizing activity of the Masc derivatives, we investigated the splicing patterns of B. mori doublesex (Bmdsx) and the expression levels of B. mori IGF-II mRNA-binding protein, a splicing regulator of Bmdsx, in Masc cDNA-transfected BmN-4 cells. We found that two zinc finger domains are not required for the masculinizing activity. We also identified that the C-terminal 288 amino acid residues are sufficient for the masculinizing activity of the Masc protein. Further detailed analyses revealed that two cysteine residues, Cys-301 and Cys-304, in the highly conserved region among lepidopteran Masc proteins are essential for the masculinizing activity in BmN-4 cells. Finally, we showed that Masc is a nuclear protein, but its nuclear localization is not tightly associated with the masculinizing activity.
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