ArticleMolecular & cellular proteomics : MCP2015
Multiple Reaction Monitoring Enables Precise Quantification of 97 Proteins in Dried Blood Spots.
Article in Molecular & cellular proteomics : MCP, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers, 1 of them a synthesis that pooled it.
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Who cites it
34 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Dried Blood Spots to Assess Cardiovascular-Kidney-Metabolic Health.Journal of the American Heart Association · 2025Pooled it
- Quantitative protein analysis and glycan profiling of umbilical cord plasma and corresponding maternal plasma samples.Scientific reports · 2026Article
- Advances in Multiple Reaction Monitoring (MRM) for Targeted Proteomics in Biomedical Research.Advances in experimental medicine and biology · 2026Review
- Proteomic signature of aging in bloodstain samples: a preliminary study.BMC genomics · 2025Article
- Quantitative Assessment of a Novel Device Designed for Patient-centric Sampling of Dried Plasma Using Targeted Proteomics.Analytical chemistry · 2025Article
- From blood drops to biomarkers: a scoping review of microsampling in mass spectrometry-based proteomics.Clinical proteomics · 2025Review
- Dried Spot Paradigm: Problems and Prospects in Proteomics.International journal of molecular sciences · 2025Review
- Use of a Novel Whole Blood Separation and Transport Device for Targeted and Untargeted Proteomics.Biomedicines · 2024Article
- Recent developments in mass-spectrometry-based targeted proteomics of clinical cancer biomarkers.Clinical proteomics · 2024Review
- Automated proteomic sample preparation: The key component for high throughput and quantitative mass spectrometry analysis.Mass spectrometry reviews · 2023Review
- Direct Processing and Storage of Cell-Free Plasma Using Dried Plasma Spot Cards.ACS measurement science au · 2022Article
- Article
- Analytical Considerations of Large-Scale Aptamer-Based Datasets for Translational Applications.Cancers · 2022Review
- Review of Liquid Chromatography-Mass Spectrometry-Based Proteomic Analyses of Body Fluids to Diagnose Infectious Diseases.International journal of molecular sciences · 2022Review
- Genome-wide postnatal changes in immunity following fetal inflammatory response.The FEBS journal · 2021Article
- Robust Microflow LC-MS/MS for Proteome Analysis: 38 000 Runs and Counting.Analytical chemistry · 2021Article
- An LC-MRM assay for the quantification of metanephrines from dried blood spots for the diagnosis of pheochromocytomas and paragangliomas.Analytica chimica acta · 2020Article
- The preventive strategy for pandemics in the elderly is to collect in advance samples & data to counteract chronic inflammation (inflammaging).Ageing research reviews · 2020Review
- Concentration Determination of >200 Proteins in Dried Blood Spots for Biomarker Discovery and Validation.Molecular & cellular proteomics : MCP · 2020Article
- Robust, reproducible and quantitative analysis of thousands of proteomes by micro-flow LC-MS/MS.Nature communications · 2020Article
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The dried blood spot (DBS) methodology provides a minimally invasive approach to sample collection and enables room-temperature storage for most analytes. DBS samples have successfully been analyzed by liquid chromatography multiple reaction monitoring mass spectrometry (LC/MRM-MS) to quantify a large range of small molecule biomarkers and drugs; however, this strategy has only recently been explored for MS-based proteomics applications. Here we report the development of a highly multiplexed MRM assay to quantify endogenous proteins in human DBS samples. This assay uses matching stable isotope-labeled standard peptides for precise, relative quantification, and standard curves to characterize the analytical performance. A total of 169 peptides, corresponding to 97 proteins, were quantified in the final assay with an average linear dynamic range of 207-fold and an average R(2) value of 0.987. The total range of this assay spanned almost 5 orders of magnitude from serum albumin (P02768) at 18.0 mg/ml down to cholinesterase (P06276) at 190 ng/ml. The average intra-assay and inter-assay precision for 6 biological samples ranged from 6.1-7.5% CV and 9.5-11.0% CV, respectively. The majority of peptide targets were stable after 154 days at storage temperatures from -20 °C to 37 °C. Furthermore, protein concentration ratios between matching DBS and whole blood samples were largely constant (<20% CV) across six biological samples. This assay represents the highest multiplexing yet achieved for targeted protein quantification in DBS samples and is suitable for biomedical research applications.
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