ArticleThe Plant cell2015
ESCRT-III-Associated Protein ALIX Mediates High-Affinity Phosphate Transporter Trafficking to Maintain Phosphate Homeostasis in Arabidopsis.
Article in The Plant cell, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 37 papers.
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Who cites it
37 citing papers in PubMed, 93 citations in OpenAlex.
- Beyond parallel pathways: molecular integration and functional crosstalk between autophagy and endocytosis in plants.Protoplasma · 2026Review
- Genome-Wide Identification of SNF7 Gene Family in Maize and Potential Roles in Response to Abiotic and Biotic Stress.International journal of molecular sciences · 2026Article
- Proteinoid Computing on Olivine Substrates.Langmuir : the ACS journal of surfaces and colloids · 2026Article
- Brassinosteroid Signaling Dynamics: Ubiquitination-Dependent Regulation of Core Signaling Components.International journal of molecular sciences · 2025Review
- The green ESCRTs: Newly defined roles for ESCRT proteins in plants.The Journal of biological chemistry · 2025Review
- Exosome-mediated Crosstalk in the Tumor Immune Microenvironment: Critical Drivers of Hepatocellular Carcinoma Progression.Journal of clinical and translational hepatology · 2025Review
- Extracellular vesicles of Norway spruce contain precursors and enzymes for lignin formation and salicylic acid.Plant physiology · 2024Article
- Arabidopsis CaLB1 undergoes phase separation with the ESCRT protein ALIX and modulates autophagosome maturation.Nature communications · 2024Article
- The plant-unique protein DRIF1 coordinates with sorting nexin 1 to regulate membrane protein homeostasis.The Plant cell · 2023Article
- Light and jasmonic acid coordinately regulate the phosphate responses under shade and phosphate starvation conditions in Arabidopsis.Plant direct · 2023Article
- The Bro1-like domain-containing protein, AtBro1, modulates growth and abiotic stress responses in Arabidopsis.Frontiers in plant science · 2023Article
- Plant autophagosomes mature into amphisomes prior to their delivery to the central vacuole.The Journal of cell biology · 2022Article
- An unexpected function for an ESCRT protein.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- Sulfur signaling and starvation response in Arabidopsis.iScience · 2022Review
- Plant ESCRT protein ALIX coordinates with retromer complex in regulating receptor-mediated sorting of soluble vacuolar proteins.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- Intracellular phosphate sensing and regulation of phosphate transport systems in plants.Plant physiology · 2021Review
- ESCRT components ISTL1 andLIP5 are required for tapetal function and pollen viability.The Plant cell · 2021Article
- Maize ZmPT7 regulates Pi uptake and redistribution which is modulated by phosphorylation.Plant biotechnology journal · 2020Article
- Regulation of Three Key Kinases of Brassinosteroid Signaling Pathway.International journal of molecular sciences · 2020Review
- Plant Membrane Transport Research in the Post-genomic Era.Plant communications · 2020Review
Corrections and comments
- Erratum issued
Authors and funding
12 authors at 4 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Prior to the release of their cargoes into the vacuolar lumen, sorting endosomes mature into multivesicular bodies (MVBs) through the action of ENDOSOMAL COMPLEX REQUIRED FOR TRANSPORT (ESCRT) protein complexes. MVB-mediated sorting of high-affinity phosphate transporters (PHT1) to the vacuole limits their plasma membrane levels under phosphate-sufficient conditions, a process that allows plants to maintain phosphate homeostasis. Here, we describe ALIX, a cytosolic protein that associates with MVB by interacting with ESCRT-III subunit SNF7 and mediates PHT1;1 trafficking to the vacuole in Arabidopsis thaliana. We show that the partial loss-of-function mutant alix-1 displays reduced vacuolar degradation of PHT1;1. ALIX derivatives containing the alix-1 mutation showed reduced interaction with SNF7, providing a simple molecular explanation for impaired cargo trafficking in alix-1 mutants. In fact, the alix-1 mutation also hampered vacuolar sorting of the brassinosteroid receptor BRI1. We also show that alix-1 displays altered vacuole morphogenesis, implying a new role for ALIX proteins in vacuolar biogenesis, likely acting as part of ESCRT-III complexes. In line with a presumed broad target spectrum, the alix-1 mutation is pleiotropic, leading to reduced plant growth and late flowering, with stronger alix mutations being lethal, indicating that ALIX participates in diverse processes in plants essential for their life.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.