ArticleApplied and environmental microbiology2015
Regulation of Gene Expression during the Onset of Ligninolytic Oxidation by Phanerochaete chrysosporium on Spruce Wood.
Article in Applied and environmental microbiology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed, 57 citations in OpenAlex.
- Plant Biomass Depolymerization by Oxidoreductases: Structural Characteristics, Synergy Mechanism, and Evolutionary Adaptation.Journal of agricultural and food chemistry · 2026Review
- Semiautomated Monitoring of Longitudinal Microbial Metabolic Dynamics: A Study Case for Lignin Degradation.ACS omega · 2026Article
- From the bench to the reactor: engineered filamentous fungi for biochemical and biomaterial production.Biotechnology for biofuels and bioproducts · 2025Review
- Metabolic mechanism of lignin-derived aromatics in white-rot fungi.Applied microbiology and biotechnology · 2024Review
- Structure-function characterization of two enzymes from novel subfamilies of manganese peroxidases secreted by the lignocellulose-degrading Agaricales fungi Agrocybe pediades and Cyathus striatus.Biotechnology for biofuels and bioproducts · 2024Article
- Biodegradation of Benzo[a]pyrene by a White-Rot FungusJournal of fungi (Basel, Switzerland) · 2023Article
- Genomic Diversity and Phenotypic Variation in Fungal Decomposers Involved in Bioremediation of Persistent Organic Pollutants.Journal of fungi (Basel, Switzerland) · 2023Article
- Comparative analysis of simulatedFrontiers in microbiology · 2023Article
- Comparative Analysis of Enzyme Production Patterns of Lignocellulose Degradation of Two White Rot Fungi:Biomolecules · 2022Article
- Gene family expansions and transcriptome signatures uncover fungal adaptations to wood decay.Environmental microbiology · 2021Article
- Trends and Applications of Omics Technologies to Functional Characterisation of Enzymes and Protein Metabolites Produced by Fungi.Journal of fungi (Basel, Switzerland) · 2021Review
- A Multiomic Approach to Understand HowJournal of fungi (Basel, Switzerland) · 2021Article
- Cycling in degradation of organic polymers and uptake of nutrients by a litter-degrading fungus.Environmental microbiology · 2021Article
- Article
- Degradation of Four Major Mycotoxins by Eight Manganese Peroxidases in Presence of a Dicarboxylic Acid.Toxins · 2019Article
- Degradation of antifungal anthraquinone compounds is a probable physiological role of DyP secreted by Bjerkandera adusta.AMB Express · 2019Article
- Oxidoreductases and Reactive Oxygen Species in Conversion of Lignocellulosic Biomass.Microbiology and molecular biology reviews : MMBR · 2018Review
- Dynamics of the Phanerochaete carnosa transcriptome during growth on aspen and spruce.BMC genomics · 2018Article
- Fungal lignin peroxidase does not produce the veratryl alcohol cation radical as a diffusible ligninolytic oxidant.The Journal of biological chemistry · 2018Article
- Gene Co-expression Network Reveals Potential New Genes Related to Sugarcane Bagasse Degradation inFrontiers in bioengineering and biotechnology · 2018Article
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Since uncertainty remains about how white rot fungi oxidize and degrade lignin in wood, it would be useful to monitor changes in fungal gene expression during the onset of ligninolysis on a natural substrate. We grew Phanerochaete chrysosporium on solid spruce wood and included oxidant-sensing beads bearing the fluorometric dye BODIPY 581/591 in the cultures. Confocal fluorescence microscopy of the beads showed that extracellular oxidation commenced 2 to 3 days after inoculation, coincident with cessation of fungal growth. Whole transcriptome shotgun sequencing (RNA-seq) analyses based on the v.2.2 P. chrysosporium genome identified 356 genes whose transcripts accumulated to relatively high levels at 96 h and were at least four times the levels found at 40 h. Transcripts encoding some lignin peroxidases, manganese peroxidases, and auxiliary enzymes thought to support their activity showed marked apparent upregulation. The data were also consistent with the production of ligninolytic extracellular reactive oxygen species by the action of manganese peroxidase-catalyzed lipid peroxidation, cellobiose dehydrogenase-catalyzed Fe(3+) reduction, and oxidase-catalyzed H2O2 production, but the data do not support a role for iron-chelating glycopeptides. In addition, transcripts encoding a variety of proteins with possible roles in lignin fragment uptake and processing, including 27 likely transporters and 18 cytochrome P450s, became more abundant after the onset of extracellular oxidation. Genes encoding cellulases showed little apparent upregulation and thus may be expressed constitutively. Transcripts corresponding to 165 genes of unknown function accumulated more than 4-fold after oxidation commenced, and some of them may merit investigation as possible contributors to ligninolysis.
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Registered trials
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