Evidence map›Paper›PMID 26340752›Full record

ArticlePloS one2015

Analysis of the Microbiota of Black Stain in the Primary Dentition.

Yue Li, Qian Zhang, Fangfei Zhang, Ruoxi Liu, He Liu, Feng Chen

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in PloS one, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
29citing papers in PubMed, 1 pooled it
4.7field-weighted citation impact, top 5% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

29 citing papers in PubMed, 1 synthesis or guideline pooled it, 61 citations in OpenAlex.

  1. Pooled it
  2. Trial
  3. Article
  4. Article
  5. Review
  6. Article
  7. Article
  8. Article
  9. Microorganisms · 2025
    Article
  10. Article
  11. Review
  12. Article
  13. Article
  14. Article
  15. Review
  16. Article
  17. Article
  18. Black stain and dental caries in primary teeth of Tunisian preschool children.European archives of paediatric dentistry : official journal of the European Academy of Paediatric Dentistry · 2021
    Article
  19. Observational
  20. In vitro inhibitory effect of two commercial probiotics on chromogenic actinomycetes.European archives of paediatric dentistry : official journal of the European Academy of Paediatric Dentistry · 2020
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 1 institution in 1 country.

Yue LiDepartment of Pediatric Dentistry, School of Stomatology, Peking University, Beijing, China.
Qian ZhangCentral Laboratory, School of Stomatology, Peking University, Beijing, China.
Fangfei ZhangDepartment of Pediatric Dentistry, School of Stomatology, Peking University, Beijing, China.
Ruoxi LiuCentral Laboratory, School of Stomatology, Peking University, Beijing, China.
He LiuDepartment of Pediatric Dentistry, School of Stomatology, Peking University, Beijing, China.
Feng ChenCentral Laboratory, School of Stomatology, Peking University, Beijing, China.
Peking University · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Black tooth stain is a characteristic extrinsic discoloration commonly seen on the cervical enamel following the contour of the gingiva. To investigate the relationship between black tooth stain and the oral microbiota, we used 16S rRNA gene sequencing to compare the microbial composition of dental plaque and saliva among caries-free children with and without black stain. Dental plaque and saliva, as well as black stain, were sampled from 10 children with and 15 children without black stain. Data were analyzed using the pipeline tool MOTHUR. Student's t-test was used to compare alpha diversities and the Mann-Whitney U test to compare the relative abundances of the microbial taxa. A total of 10 phyla, 19 classes, 32 orders, 61 families and 102 genera were detected in these samples. Shannon and Simpson diversity were found to be significantly lower in saliva samples of children with black stain. Microbial diversity was reduced in the black stain compared to the plaque samples. Actinomyces, Cardiobacterium, Haemophilus, Corynebacterium, Tannerella and Treponema were more abundant and Campylobacter less abundant in plaque samples of children with black stain. Principal component analysis demonstrated clustering among the dental plaque samples from the control group, while the plaque samples from the black stain group were not and appeared to cluster into two subgroups. Alterations in oral microbiota may be associated with the formation of black stain.

Indexed as

Genes, BacterialActinomycesBacteroidetesCardiobacteriumCase-Control StudiesChild, PreschoolCorynebacteriumDental PlaqueFemaleHaemophilusHumansMaleMicrobiotaMultigene FamilyPhylogenyRNA, Ribosomal, 16SRNA, Ribosomal, 16S

Identifiers

PMID26340752
PMCPMC4560370
OpenAlexW2137499886

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.