ArticleBioMed research international2015
ZnO Nanoparticles Treatment Induces Apoptosis by Increasing Intracellular ROS Levels in LTEP-a-2 Cells.
Article in BioMed research international, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed.
- Effect of Zinc Oxide Nanoparticles From Infant Formula Milk Powder on Colon Cells In Vitro.Journal of applied toxicology : JAT · 2026Article
- Flower-shaped ZnO nanoparticles from Lactococcus lactis LAB2: potential selective anti-cancer activity via oxidative stress modulation.World journal of microbiology & biotechnology · 2026Article
- Biogenic synthesis of zinc oxide nanoparticles usingBiotechnology notes (Amsterdam, Netherlands) · 2026Article
- Targeting metalloptosis in tumor therapy: from molecular mechanisms to application of metal nanoparticles.Molecular cancer · 2025Review
- Peony-shaped zinc oxide nanoflower synthesized via hydrothermal route exhibits promising anticancer and anti-amyloid activity.BMC pharmacology & toxicology · 2024Article
- Anticancer activity of zinc oxide nanoparticles on prostate and colon cancer cell line.Toxicology research · 2024Article
- Biosynthesis of zinc oxide nanoparticlesPeerJ · 2024Article
- Zinc Oxide Nanoparticles Promise Anticancer and Antibacterial Activity in Ovarian Cancer.Pharmaceutical research · 2023Article
- Zinc Oxide Nanoparticles (ZnO-NPs) Induce Cytotoxicity in the Zebrafish Olfactory Organs via Activating Oxidative Stress and Apoptosis at the Ultrastructure and Genetic Levels.Animals : an open access journal from MDPI · 2023Article
- Method for nanoparticles uptake evaluation based on double labeled fluorescent cells scanned in enhanced darkfield microscopy.Biomedical optics express · 2023Article
- Biosynthesis and Antimicrobial Evaluation of Zinc Oxide Nanoparticles UsingMaterials (Basel, Switzerland) · 2023Article
- Biogenic Zinc oxide nanoparticles fromFrontiers in bioengineering and biotechnology · 2023Article
- Effects of green synthesized zinc nanoparticles alone and along with albendazole against hydatid cyst protoscoleces.Annals of medicine and surgery (2012) · 2022Article
- Histological and biochemical apoptosis changes of female rats' ovary by Zinc oxide nanoparticles and potential protective effects of l-arginine: An experimental study.Annals of medicine and surgery (2012) · 2022Article
- Effect of UV Irradiation (A and C) onPharmaceutics · 2021Article
- Functionalized Scintillating Nanotubes for Simultaneous Radio- and Photodynamic Therapy of Cancer.ACS applied materials & interfaces · 2021Article
- Genotoxic and Cytotoxic Properties of Zinc Oxide Nanoparticles Phyto-Fabricated from the Obscure Morning Glory PlantMolecules (Basel, Switzerland) · 2021Article
- Metal Oxide Nanoparticles as Biomedical Materials.Biomimetics (Basel, Switzerland) · 2020Review
- The Impact of Zinc Oxide Nanoparticles on Male (In)Fertility.Materials (Basel, Switzerland) · 2020Review
- Carbon-dot wrapped ZnO nanoparticle-based photoelectrochemical sensor for selective monitoring of HMikrochimica acta · 2019Article
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4 authors.
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Abstract
Owing to the wide use of novel nanoparticles (NPs) such as zinc oxide (ZnO) in all aspects of life, toxicological research on ZnO NPs is receiving increasing attention in these days. In this study, the toxicity of ZnO NPs in a human pulmonary adenocarcinoma cell line LTEP-a-2 was tested in vitro. Log-phase cells were exposed to different levels of ZnO NPs for hours, followed by colorimetric cell viability assay using tetrazolium salt and cell survival rate assay using trypan blue dye. Cell morphological changes were observed by Giemsa staining and light microscopy. Apoptosis was detected by using fluorescence microscopy and caspase-3 activity assay. Both intracellular reactive oxygen species (ROS) and reduced glutathione (GSH) were examined by a microplate-reader method. Results showed that ZnO NPs (≥ 0.01 μg/mL) significantly inhibited proliferation (P < 0.05) and induced substantial apoptosis in LTEP-a-2 cells after 4 h of exposure. The intracellular ROS level rose up to 30-40% corresponding to significant depletion (approximately 70-80%) in GSH content in LTEP-a-2 cells (P < 0.05), suggesting that ZnO NPs induced apoptosis mainly through increased ROS production. This study elucidates the toxicological mechanism of ZnO NPs in human pulmonary adenocarcinoma cells and provides reference data for application of nanomaterials in the environment.
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