ArticleBioMed research international2015
Oxidative Stress Alters miRNA and Gene Expression Profiles in Villous First Trimester Trophoblasts.
Article in BioMed research international, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers, 1 of them a synthesis that pooled it.
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Who cites it
26 citing papers in PubMed, 1 synthesis or guideline pooled it, 45 citations in OpenAlex.
- Systematic Review of Micro-RNA Expression in Pre-Eclampsia Identifies a Number of Common Pathways Associated with the Disease.PloS one · 2016Pooled it
- Aberrant Uteroplacental and Vascular Signaling and Remodeling by Matrix Metalloproteinases in Pregnancy-Related Hypertension and Preeclampsia.Biomolecules · 2026Review
- MiR-425-5p suppression of Crebzf regulates oocyte aging via chromatin modification.GeroScience · 2024Article
- Review
- Assessment of Oxidative Stress-Induced Oral Epithelial Toxicity.Biomolecules · 2023Article
- High molecular weight hyaluronic acid drastically reduces chemotherapy-induced mucositis and apoptotic cell death.Cell death & disease · 2023Article
- Tianma Gouteng Decoction Exerts Pregnancy-Protective Effects Against PreeclampsiaFrontiers in pharmacology · 2022Article
- The Anticancer Effects of Flavonoids through miRNAs Modulations in Triple-Negative Breast Cancer.Nutrients · 2021Review
- Age and Sex-Related Changes in Human First-Trimester Placenta Transcriptome and Insights into Adaptative Responses to Increased Oxygen.International journal of molecular sciences · 2021Article
- Appropriate citation of placenta cell lines 3A(tPA-30-1) and 3A-sub E [post crisis of 3A(tPA-30-1)] in medical literature.Heliyon · 2020Article
- Vascular mechanisms and molecular targets in hypertensive pregnancy and preeclampsia.American journal of physiology. Heart and circulatory physiology · 2020Review
- Placenta Disrupted: Endocrine Disrupting Chemicals and Pregnancy.Trends in endocrinology and metabolism: TEM · 2020Review
- ROS/p53/miR‑335‑5p/Sp1 axis modulates the migration and epithelial to mesenchymal transition of JEG‑3 cells.Molecular medicine reports · 2020Article
- Preeclampsia: The Interplay Between Oxygen-Sensitive miRNAs and Erythropoietin.Journal of clinical medicine · 2020Article
- Crosstalk of MicroRNAs and Oxidative Stress in the Pathogenesis of Cancer.Oxidative medicine and cellular longevity · 2020Review
- Critical Role of Oxidatively Damaged DNA in Selective Noradrenergic Vulnerability.Neuroscience · 2019Article
- Novel biomarker profiles in experimental aged maternal mice with hypertensive disorders of pregnancy.Hypertension research : official journal of the Japanese Society of Hypertension · 2019Article
- Molecular determinants of microvascular dysfunction in hypertensive pregnancy and preeclampsia.Microcirculation (New York, N.Y. : 1994) · 2018Article
- Long Noncoding RNA 00473 Is Involved in Preeclampsia by LSD1 Binding-Regulated TFPI2 Transcription in Trophoblast Cells.Molecular therapy. Nucleic acids · 2018Article
- Early Detection of Preeclampsia Using Circulating Small non-coding RNA.Scientific reports · 2018Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
Abstract
The relationship between oxidative stress and miRNA changes in placenta as a potential mechanism involved in preeclampsia (PE) is not fully elucidated. We investigated the impact of oxidative stress on miRNAs and mRNA expression profiles of genes associated with PE in villous 3A first trimester trophoblast cells exposed to H2O2 at 12 different concentrations (0-1 mM) for 0.5, 4, 24, and 48 h. Cytotoxicity, determined using the SRB assay, was used to calculate the IC50 of H2O2. RNA was extracted after 4 h exposure to H2O2 for miRNA and gene expression profiling. H2O2 exerted a concentration- and time-dependent cytotoxicity on 3A trophoblast cells. Short-term exposure of 3A cells to low concentration of H2O2 (5% of IC50) significantly altered miRNA profile as evidenced by significant changes in 195 out of 595 evaluable miRNAs. Tool for annotations of microRNAs (TAM) analysis indicated that these altered miRNAs fall into 43 clusters and 34 families, with 41 functions identified. Exposure to H2O2 altered mRNA expression of 22 out of 84 key genes involved in dysregulation of placental development. In conclusion, short-term exposure of villous first trimester trophoblasts to low concentrations of H2O2 significantly alters miRNA profile and expression of genes implicated in placental development.
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