Evidence map›Paper›PMID 26339381›Full record

ArticleInternational journal of clinical and experimental pathology2015

Retinoic acid induced the differentiation of neural stem cells from embryonic spinal cord into functional neurons in vitro.

Bo-Tao Tan, Li Wang, Sen Li, Zai-Yun Long, Ya-Min Wu, Yuan Liu

Open access · greenAbstract read
PubMed Full text at PMC
In one paragraph

Article in International journal of clinical and experimental pathology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.

0numbers the graph read from it
0cells of the map it votes in
18citing papers in PubMed
0.9field-weighted citation impact, top 28% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

18 citing papers in PubMed, 32 citations in OpenAlex.

  1. Article
  2. Article
  3. Article
  4. Review
  5. Article
  6. Article
  7. Article
  8. Neurogenesis as a Tool for Spinal Cord Injury.International journal of molecular sciences · 2022
    Review
  9. Article
  10. Article
  11. Peptide Regulation of Cell Differentiation.Stem cell reviews and reports · 2020
    Review
  12. Article
  13. Review
  14. Review
  15. Article
  16. Article
  17. Article
  18. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 1 institution in 1 country.

Bo-Tao TanThe 3rd Department of Research Institute of Surgery, Daping Hospital, The Third Military Medical University, State Key Laboratory of Trauma, Burns and Combined Injury Chongqing 400042, PR China.
Li WangThe 3rd Department of Research Institute of Surgery, Daping Hospital, The Third Military Medical University, State Key Laboratory of Trauma, Burns and Combined Injury Chongqing 400042, PR China.
Sen LiThe 3rd Department of Research Institute of Surgery, Daping Hospital, The Third Military Medical University, State Key Laboratory of Trauma, Burns and Combined Injury Chongqing 400042, PR China.
Zai-Yun LongThe 3rd Department of Research Institute of Surgery, Daping Hospital, The Third Military Medical University, State Key Laboratory of Trauma, Burns and Combined Injury Chongqing 400042, PR China.
Ya-Min WuThe 3rd Department of Research Institute of Surgery, Daping Hospital, The Third Military Medical University, State Key Laboratory of Trauma, Burns and Combined Injury Chongqing 400042, PR China.
Yuan LiuThe 3rd Department of Research Institute of Surgery, Daping Hospital, The Third Military Medical University, State Key Laboratory of Trauma, Burns and Combined Injury Chongqing 400042, PR China.
Army Medical University · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Retinoic acid is an important molecular taking part in the development and homeostasis of nervous system. Neural stem cells (NSCs) are pluripotent cells that can differentiate into three main neural cells including neuron, astrocyte and oligodendrocyte. However, whether retinoic acid can induce NSCs derived from embryonic spinal cord differentiating into functional neurons and its efficiency are not clear. In this experiment, NSCs were isolated from embryonic 14 d spinal cord of rats. The growth and neuronal differentiation of NSCs induced by 500 nM RA was examined in vitro. It was indicated that compared with the control group, there were more differentiated cells with longer cytodendrites in the medium treated with RA at different time. And more, there were more neuronal marker positive cells in 500 nM RA group than the control group seven days after differentiation. At the same time, the expression of β-tublin III protein in RA group was higher than those in control group, which was contrary to the expression of astrocyte marker GFAP protein at seven days after differentiation. However the differentiated neurons, whether treated with RA or not both exhibited biological electrical reactivity after stimulated by glutamine. Therefore, these findings indicated that RA could promote growth of cellular dendrites and neuronal differentiation of NSCs, which also induce functional maturation of differentiated neurons finally.

Indexed as

AnimalsBiomarkersCalciumCells, CulturedGestational AgeGlial Fibrillary Acidic ProteinGlutamineMembrane PotentialsNeural Stem CellsNeurogenesisRats, WistarSpinal CordTime FactorsTretinoinTubulinBiomarkersCalciumGlial Fibrillary Acidic ProteinGlutamineTretinoinTubb3 protein, ratTubulinculturedifferentiationneural stem cellsRetinoic acid

Identifiers

PMID26339381
PMCPMC4555709
OpenAlexW1480005003

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.