ArticleBiomicrofluidics2015
Microfluidic assessment of swimming media for motility-based sperm selection.
Article in Biomicrofluidics, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
24 citing papers in PubMed, 49 citations in OpenAlex.
- From reservoir to rendezvous: the journey of sperm through the oviduct.Reproduction, fertility, and development · 2026Review
- Deep learning-based morphological analysis of human sperm.Medical & biological engineering & computing · 2026Article
- Simple and rapid microfluidic device for processing raw human semen and separation of motile sperm based on their intrinsic behavior and morphology.Scientific reports · 2025Article
- Open-Channel Droplet Microfluidic Platform for Passive Generation of Human Sperm Microdroplets.ACS biomaterials science & engineering · 2025Article
- Open-Channel Droplet Microfluidic Platform for Passive Generation of Human Sperm Microdroplets.bioRxiv : the preprint server for biology · 2024Article
- Fallopian tube rheology regulates epithelial cell differentiation and function to enhance cilia formation and coordination.Nature communications · 2024Article
- Sperm in the Mammalian Female Reproductive Tract: Surfing Through the Tract to Try to Beat the Odds.Annual review of animal biosciences · 2024Review
- Revolutionizing the female reproductive system research using microfluidic chip platform.Journal of nanobiotechnology · 2023Review
- Sperm quality metrics were improved by a biomimetic microfluidic selection platform compared to swim-up methods.Microsystems & nanoengineering · 2023Article
- Effect of High Viscosity on Energy Metabolism and Kinematics of Spermatozoa from Three Mouse Species Incubated under Capacitating Conditions.International journal of molecular sciences · 2022Article
- The influence of the female reproductive tract and sperm features on the design of microfluidic sperm-sorting devices.Journal of assisted reproduction and genetics · 2022Review
- A Sperm Quality Detection System Based on Microfluidic Chip and Micro-Imaging System.Frontiers in veterinary science · 2022Article
- An Update on Semen Physiology, Technologies, and Selection Techniques for the Advancement of In Vitro Equine Embryo Production: Section II.Animals : an open access journal from MDPI · 2021Review
- Viscous Cervical Environment-on-a-Chip for Selecting High-Quality Sperm from Human Semen.Biomedicines · 2021Article
- Rheotaxis-based sperm separation using a biomimicry microfluidic device.Scientific reports · 2021Article
- Self-adaptive and efficient propulsion of Ray sperms at different viscosities enabled by heterogeneous dual helixes.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- A microfluidic approach to rapid sperm recovery from heterogeneous cell suspensions.Scientific reports · 2021Article
- A PMMA-Based Microfluidic Device for Human Sperm Evaluation and Screening on Swimming Capability and Swimming Persistence.Micromachines · 2020Article
- The motility-based swim-up technique separates bull sperm based on differences in metabolic rates and tail length.PloS one · 2019Article
- Migration of magnetotactic bacteria in porous media.Biomicrofluidics · 2018Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Selection medium is important in sperm isolation for assisted reproductive technologies. Contrary to the naturally occurring human cervical mucus which has a high viscosity, most current practices for motility based sperm selection use a low viscosity medium. In this study, we used a microfluidic device to assess the effects of high viscosity media made with hyaluronic acid (HA) and methyl cellulose (MC) on bovine and human sperm motility and viability (sperm transferred directly from cryoprotectant). The microfluidic penetration test, viability, and motility were compared for sperm swimming in both HA and MC media with about 20cp viscosity (measured at 20 °C). Our resulted indicate that MC medium resulted in a significantly higher number of viable bovine sperm penetrating the medium as compared to HA. Furthermore, MC resulted in the selection of a sperm subpopulation with a 274% increase in sperm viability in comparison to the raw semen, while HA increased viability by only 133%. In addition to viability, bovine sperm motility parameters were significantly higher in the MC medium as compared with HA. Experiments with human sperm swimming in MC indicate that sperm swim slower and straighter at higher viscosities. In conclusion, the results indicate that in a micro-confined environment representative of the in vivo environment, MC is a preferred high viscosity medium to ensure the highest concentration of motile and viable sperm.
Identifiers
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.