Evidence map›Paper›PMID 26339064›Full record

ArticleJournal of virology2015

Preferential Budding of Vesicular Stomatitis Virus from the Basolateral Surface of Polarized Epithelial Cells Is Not Solely Directed by Matrix Protein or Glycoprotein.

Eugene Drokhlyansky, Timothy K Soh, Constance L Cepko

Abstract read
In one paragraph

Article in Journal of virology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed.

  1. Review
  2. Article
  3. Review
  4. Article
  5. The brain parenchyma has a type I interferon response that can limit virus spread.Proceedings of the National Academy of Sciences of the United States of America · 2017
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

Eugene DrokhlyanskyDepartment of Genetics and Department of Ophthalmology, Harvard Medical School, Howard Hughes Medical Institute, Boston, Massachusetts, USA.
Timothy K SohDepartment of Microbiology and Immunobiology, Program in Virology, Harvard Medical School, Boston, Massachusetts, USA.
Constance L CepkoDepartment of Genetics and Department of Ophthalmology, Harvard Medical School, Howard Hughes Medical Institute, Boston, Massachusetts, USA cepko@genetics.med.harvard.edu.

Funding

STRUCTURE-FUNCTION RELATIONSHIPS IN THE ALIMENTARY TRACTP30DK034854 · NIDDK · HARVARD UNIVERSITY (MEDICAL SCHOOL) · PI WAYNE I LENCER · 1986 to 2026
$32.4M
Development of novel transsynaptic tracers for use in the central nervous systemR01NS083848 · NINDS · HARVARD MEDICAL SCHOOL · PI CEPKO, CONSTANCE L · 2013 to 2017
$1.8M
The role of p97 in protein degradation during aging and diseaseF31AG041582 · NIA · HARVARD MEDICAL SCHOOL · PI DROKHLYANSKY, EUGENE · 2011 to 2013
$92k
NIA NIH HHS F31 AG041582NIDDK NIH HHS P30 DK034854NINDS NIH HHS R01 NS083848
6 · The paper itself

Abstract

Vesicular stomatitis virus has been shown to bud basolaterally, and the matrix protein, but not glycoprotein, was proposed to mediate this asymmetry. Using polarized T84 monolayers, we demonstrate that no single viral protein is sufficient for polarized budding. Particles are released from the apical and basolateral surfaces and are indistinguishable, indicating that there is no apical assembly defect. We propose that aspects of host cell polarity create a more efficient budding process at the basolateral surface.

Indexed as

Cell LineCell PolarityEpithelial CellsGlycoproteinsVesicular stomatitis Indiana virusViral Envelope ProteinsViral Matrix ProteinsVirus ReleaseGlycoproteinsViral Envelope ProteinsViral Matrix Proteins

Identifiers

PMID26339064
PMCPMC4645653

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.