ArticleProtoplasma2016
NO is involved in spermidine-induced drought tolerance in white clover via activation of antioxidant enzymes and genes.
Article in Protoplasma, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 57 citations in OpenAlex.
- PagSAMDC4a-Mediated Polyamine Synthesis Regulate Vessel Differentiation Under Drought Stress Conditions in Poplar.Plant biotechnology journal · 2025Article
- Polyamines Interaction with Gaseous Signaling Molecules for Resilience Against Drought and Heat Stress in Plants.Plants (Basel, Switzerland) · 2025Review
- Nitric oxide regulates photosynthesis and the capacity of the antioxidant system under water deficit and rehydration inFrontiers in plant science · 2025Article
- Polyamines: pleiotropic molecules regulating plant development and enhancing crop yield and quality.Plant biotechnology journal · 2024Review
- Mechanism of action of microRNA166 on nitric oxide in alfalfa (Medicago sativa L.) under drought stress.BMC genomics · 2024Article
- Polyamines in Edible and Medicinal Fungi from Serbia: A Novel Perspective on Neuroprotective Properties.Journal of fungi (Basel, Switzerland) · 2023Article
- The Plant Metabolic Changes and the Physiological and Signaling Functions in the Responses to Abiotic Stress.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Salvianolic Acid Modulates Physiological Responses and Stress-Related Genes That Affect Osmotic Stress Tolerance inFrontiers in plant science · 2022Article
- Plant Nitric Oxide Signaling under Drought Stress.Plants (Basel, Switzerland) · 2021Review
- Nitric Oxide Signal, Nitrogen Metabolism, and Water Balance Affected by γ-Aminobutyric Acid (GABA) in Relation to Enhanced Tolerance to Water Stress in Creeping Bentgrass.International journal of molecular sciences · 2020Article
- Exogenously applied proline induced changes in key anatomical features and physio-biochemical attributes in water stressed oat (Physiology and molecular biology of plants : an international journal of functional plant biology · 2019Article
- Polyamines and Legumes: Joint Stories of Stress, Nitrogen Fixation and Environment.Frontiers in plant science · 2019Review
- Comparative proteomic investigation of drought responses in foxtail millet.BMC plant biology · 2018Article
- Reactive oxygen species and nitric oxide are involved in polyamine-induced growth inhibition in wheat plants.Protoplasma · 2018Article
- Over-Expression of a MaizeFrontiers in plant science · 2018Article
- Protection of Pepper Plants from Drought byFrontiers in microbiology · 2018Article
- Xanthine dehydrogenase-1 silencing inFASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2017Article
- Interaction of Polyamines, Abscisic Acid, Nitric Oxide, and Hydrogen Peroxide under Chilling Stress in Tomato (Frontiers in plant science · 2017Article
- Transgenic Centipedegrass (Frontiers in plant science · 2017Article
- Differential Responses of Polyamines and Antioxidants to Drought in a Centipedegrass Mutant in Comparison to Its Wild Type Plants.Frontiers in plant science · 2017Article
Corrections and comments
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Authors and funding
11 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Nitric oxide (NO), a key signaling molecule, can be induced by polyamines (PAs), which play an important role in improving drought tolerance in plants. This study was to further investigate the role of NO in spermidine (Spd)-induced drought tolerance associated with antioxidant defense in leaves of white clover (Trifolium repens) under drought stress induced by -0.3 MPa polyethylene glycol (PEG-6000) solution. A hydroponic growth method was used for cultivating plants in a controlled growth chamber for 30-33 days until the second leaves were fully expanded. Two relative independent experiments were carried out in our study. One is that exogenous application of Spd or an NO donor (sodium nitroprusside (SNP)) significantly improved drought tolerance in whole plants, as demonstrated by better phenotypic appearance, increased relative water content (RWC), and decreased electrolyte leakage (EL) and malondialdehyde (MDA) content in leaves as compared to untreated plants. For another detached leaf experiment, PEG induced an increase in the generation of NO in cells and significantly improved activities of nitrate reductase (NR) and nitric oxide synthase (NOS). These responses could be blocked by pre-treatment with a Spd biosynthetic inhibitor, dicyclohexyl amine (DCHA), and then reversed by application of exogenous Spd. Meanwhile, PEG induced up-regulation of activities and gene transcript levels of corresponding antioxidant enzymes, including superoxide dismutase (SOD), catalase (CAT), peroxidase (POD), and ascorbate peroxidase (APX) to varying degrees, while these effects were partially blocked by pre-treatment with DCHA, the scavenger of NO, the inhibitors of NR or NOS. In addition, Spd-induced antioxidant enzyme activities and gene expression also could be effectively inhibited by an NO scavenger as well as inhibitors of NR and NOS. These findings suggest that both Spd and NO can enhance drought tolerance. Spd was involved in drought stress-activated NR and NOS pathways associated with NO release, which mediated antioxidant defense and thus contributed to drought tolerance in white clover.
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