ArticlePlant molecular biology2015
Tissue culture-induced genetic and epigenetic variation in triticale (× Triticosecale spp. Wittmack ex A. Camus 1927) regenerants.
Article in Plant molecular biology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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Who cites it
30 citing papers in PubMed.
- β-glucans, SAM, and GSH fluctuations in barley anther tissue culture conditions affect regenerants' DNA methylation and GPRE.BMC plant biology · 2024Article
- Constitutive overexpression ofFrontiers in plant science · 2024Article
- Generating homozygous mutant populations of barley microspores by ethyl methanesulfonate treatment.aBIOTECH · 2023Article
- Exploring Genetic and Epigenetic Changes in Lingonberry Using Molecular Markers: Implications for Clonal Propagation.Current issues in molecular biology · 2023Article
- Article
- Triticale doubled haploid plant regeneration factors linked by structural equation modeling.Journal of applied genetics · 2022Article
- Effect of copper and silver ions on sequence and DNA methylation changes in triticale regenerants gained via somatic embryogenesis.Journal of applied genetics · 2022Article
- Applications of In Vitro Tissue Culture Technologies in Breeding and Genetic Improvement of Wheat.Plants (Basel, Switzerland) · 2022Review
- Article
- Cultivar-specific markers, mutations, and chimerisim of Cavendish banana somaclonal variants resistant to Fusarium oxysporum f. sp. cubense tropical race 4.BMC genomics · 2022Article
- Quantitative Trait Loci and Candidate Genes Associated with Cold-Acclimation andPlants (Basel, Switzerland) · 2021Article
- Article
- Understanding In Vitro Tissue Culture-Induced Variation Phenomenon in Microspore System.International journal of molecular sciences · 2021Review
- Comparative Study of the Genetic and Biochemical Variability ofInternational journal of molecular sciences · 2021Article
- Barley somatic embryogenesis-an attempt to modify variation induced in tissue culture.Journal of biological research (Thessalonike, Greece) · 2021Article
- Applications of Doubled Haploids in Plant Breeding and Applied Research.Methods in molecular biology (Clifton, N.J.) · 2021Review
- Time of In Vitro Anther Culture May Moderate Action of Copper and Silver Ions that Affect the Relationship between DNA Methylation Change and the Yield of Barley Green Regenerants.Plants (Basel, Switzerland) · 2020Article
- Exploring the Biochemical Origin of DNA Sequence Variation in Barley Plants Regenerated via in Vitro Anther Culture.International journal of molecular sciences · 2020Article
- Plant Genome Editing and the Relevance of Off-Target Changes.Plant physiology · 2020Article
- CG Demethylation Leads to Sequence Mutations in an Anther Culture of Barley Due to the Presence of Cu, Ag Ions in the Medium and Culture Time.International journal of molecular sciences · 2020Article
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3 authors.
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Abstract
Plant regeneration via in vitro culture can induce genetic and epigenetic variation; however, the extent of such changes in triticale is not yet understood. In the present study, metAFLP, a variation of methylation-sensitive amplified fragment length polymorphism analysis, was used to investigate tissue culture-induced variation in triticale regenerants derived from four distinct genotypes using androgenesis and somatic embryogenesis. The metAFLP technique enabled identification of both sequence and DNA methylation pattern changes in a single experiment. Moreover, it was possible to quantify subtle effects such as sequence variation, demethylation, and de novo methylation, which affected 19, 5.5, 4.5% of sites, respectively. Comparison of variation in different genotypes and with different in vitro regeneration approaches demonstrated that both the culture technique and genetic background of donor plants affected tissue culture-induced variation. The results showed that the metAFLP approach could be used for quantification of tissue culture-induced variation and provided direct evidence that in vitro plant regeneration could cause genetic and epigenetic variation.
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