ArticleConnective tissue research2016
Synergistic enhancement of ectopic bone formation by supplementation of freshly isolated marrow cells with purified MSC in collagen-chitosan hydrogel microbeads.
Article in Connective tissue research, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed.
- Review
- Conductive extracellular matrix derived/chitosan methacrylate/ graphene oxide-pegylated hybrid hydrogel for cell expansion.Frontiers in bioengineering and biotechnology · 2024Article
- Hydrogel Microparticles for Bone Regeneration.Gels (Basel, Switzerland) · 2023Review
- Synthesis, Characterization and Biological Properties of Type I Collagen-Chitosan Mixed Hydrogels: A Review.Gels (Basel, Switzerland) · 2023Review
- A Conceptual Approach for Examining Effects of the Adolescent Bone Marrow Milieu on MSC Phenotype.JBMR plus · 2023Article
- New Challenges and Prospective Applications of Three-Dimensional Bioactive Polymeric Hydrogels in Oral and Craniofacial Tissue Engineering: A Narrative Review.Pharmaceuticals (Basel, Switzerland) · 2023Review
- Multimodular vascularized bone construct comprised of vasculogenic and osteogenic microtissues.Biotechnology and bioengineering · 2022Article
- Coupling Osteogenesis and Vasculogenesis in Engineered Orthopedic Tissues.Tissue engineering. Part B, Reviews · 2021Review
- Development of a modular, biocompatible thiolated gelatin microparticle platform for drug delivery and tissue engineering applications.Regenerative biomaterials · 2021Article
- Microfluidics generation of chitosan microgels containing glycerylphytate crosslinker for in situ human mesenchymal stem cells encapsulation.Materials science & engineering. C, Materials for biological applications · 2021Article
- Modular microcarrier technologies for cell-based bone regeneration.Journal of materials chemistry. B · 2020Review
- Hydrogel microparticles for biomedical applications.Nature reviews. Materials · 2020Article
- Injectable osteogenic microtissues containing mesenchymal stromal cells conformally fill and repair critical-size defects.Biomaterials · 2019Article
- Microgels: Modular, tunable constructs for tissue regeneration.Acta biomaterialia · 2019Review
- Bioactive hydrogels for bone regeneration.Bioactive materials · 2018Review
- Biofabrication of injectable fibrin microtissues for minimally-invasive therapies: application of surfactants.Biomedical materials (Bristol, England) · 2018Article
- Secretome released from hydrogel-embedded adipose mesenchymal stem cells protects against the Parkinson's disease related toxin 6-hydroxydopamine.European journal of pharmaceutics and biopharmaceutics : official journal of Arbeitsgemeinschaft fur Pharmazeutische Verfahrenstechnik e.V · 2017Article
- In-vivo evaluation of subcutaneously implanted cell-loaded apatite microcarriers for osteogenic potency.Journal of materials science. Materials in medicine · 2017Article
- The mechanism of a chitosan-collagen composite film used as biomaterial support for MC3T3-E1 cell differentiation.Scientific reports · 2016Article
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4 authors.
Funding
Abstract
purposeBone marrow-derived mesenchymal stem cells (MSC) can differentiate osteogenic lineages, but their tissue regeneration ability is inconsistent. The bone marrow mononuclear cell (BMMC) fraction of adult bone marrow contains a variety of progenitor cells that may potentiate tissue regeneration. This study examined the utility of BMMC, both alone and in combination with purified MSC, as a cell source for bone regeneration.
methodsFresh BMMC, culture-expanded MSC, and a combination of BMMC and MSC were encapsulated in collagen-chitosan hydrogel microbeads for pre-culture and minimally invasive delivery. Microbeads were cultured in growth medium for 3 days, and then in either growth or osteogenic medium for 17 days prior to subcutaneous injection in the rat dorsum.
resultsMSC remained viable in microbeads over 17 days in pre-culture, while some of the BMMC fraction were nonviable. After 5 weeks of implantation, microCT and histology showed that supplementation of BMMC with MSC produced a strong synergistic effect on the volume of ectopic bone formation, compared to either cell source alone. Microbeads containing only fresh BMMC or only cultured MSC maintained in osteogenic medium resulted in more bone formation than their counterparts cultured in growth medium. Histological staining showed evidence of residual microbead matrix in undifferentiated samples and indications of more advanced tissue remodeling in differentiated samples.
conclusionsThese data suggest that components of the BMMC fraction can act synergistically with predifferentiated MSC to potentiate ectopic bone formation. The microbead system may have utility in delivering desired cell populations in bone regeneration applications.
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