ArticleMalaria journal2015
Implementation and application of a multiplex assay to detect malaria-specific antibodies: a promising tool for assessing malaria transmission in Southeast Asian pre-elimination areas.
Article in Malaria journal, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers, 1 of them a synthesis that pooled it.
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Who cites it
31 citing papers in PubMed, 1 synthesis or guideline pooled it, 52 citations in OpenAlex.
- Pooled it
- Geographical patterns of malaria transmission based on serological markers for falciparum and vivax malaria in Ratanakiri, Cambodia.Malaria journal · 2016Trial
- Serological surveillance for malaria elimination in Southeast Asia: a scoping review.Malaria journal · 2026Article
- Anopheles mosquito fauna, blood meal sources and transmission intensity from high and moderate malaria endemic areas of Ethiopia.Scientific reports · 2025Article
- Principal component analysis of the Serological response to Plasmodium Falciparum using a Multiplex bead-based assay in Nigeria.Scientific reports · 2024Article
- Using Serological Markers for the Surveillance ofPathogens (Basel, Switzerland) · 2023Article
- Analysis of Antibodies Induced after SARS-CoV-2 Vaccination Using Antigen Coded Bead Array Luminex Technology.Vaccines · 2023Article
- Identification of factors associated with residual malaria transmission using school-based serological surveys in settings pursuing elimination.Malaria journal · 2022Article
- Development of a Bead-Based Multiplex Assay for Use in Multianalyte Screening and Surveillance of HIV, Viral Hepatitis, Syphilis, and Herpes.Journal of clinical microbiology · 2022Article
- Surveillance of Plasmodium vivax transmission using serological models in the border areas of China-Myanmar.Malaria journal · 2022Article
- Spatial, environmental, and individual associations withFrontiers in cellular and infection microbiology · 2022Article
- Antibody dynamics in children with first or repeatFrontiers in medicine · 2022Article
- Peptide Biomarkers for the Diagnosis of Dengue Infection.Frontiers in immunology · 2022Article
- Epidemiological characterization of imported recurrent Plasmodium vivax and Plasmodium ovale in China, 2013-2020.Infectious diseases of poverty · 2021Article
- School-Based Serosurveys to Assess the Validity of Using Routine Health Facility Data to Target Malaria Interventions in the Central Highlands of Madagascar.The Journal of infectious diseases · 2021Article
- Evaluating SARS-CoV-2 spike and nucleocapsid proteins as targets for antibody detection in severe and mild COVID-19 cases using a Luminex bead-based assay.Journal of virological methods · 2021Article
- Framework for Characterizing Longitudinal Antibody Response in Children AfterFrontiers in immunology · 2021Article
- Measuring antibody avidity to Plasmodium falciparum merozoite antigens using a multiplex immunoassay approach.Malaria journal · 2020Article
- Screening for malaria antigen and anti-malarial IgG antibody in forcibly-displaced Myanmar nationals: Cox's Bazar district, Bangladesh, 2018.Malaria journal · 2020Article
- Quality control of multiplex antibody detection in samples from large-scale surveys: the example of malaria in Haiti.Scientific reports · 2020Article
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Authors and funding
11 authors at 5 institutions in 5 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundEpidemiological surveillance is a key activity in malaria control and elimination in low-transmission and pre-elimination settings. Hence, sensitive tools for estimating malaria force of infection are crucial. Serological markers might provide additional information in estimating force of infection in low-endemic areas along with classical parasite detection methods. Serological markers can be used to estimate recent, past or present malaria exposure, depending on the used markers and their half-life.
methodsAn assay based on 14 Plasmodium-specific peptides, one peptide specific for Anopheles gambiae saliva protein and five Plasmodium-specific recombinant proteins was developed for the MAGPIX system, assessed for its performance, and applied on blood spots from 2000 individuals collected in the Ratanakiri Province, Cambodia.
resultsA significant correlation for the use of 1000 and 2000 beads/antigen/well as well as for the monoplex versus multiplex assay was observed for all antigens (p < 0.05). For the majority of antigens, antigen-coupled beads were stable for at least 2 months. The assay was very reproducible with limited intercoupling, interplate and intraplate variability (mean RSD <15 %). Estimating seroconversion and seroreversion per antigen using reversible catalytic models and models allowing two seroconversion rates showed higher seroconversion rates in adults.
conclusionThe multiplex bead-based immunoassay was successfully implemented and analysis of field blood samples shows that changes detected in force of malaria infection vary according to the serological markers used. Multivariate analysis of the antibody responses and insights into the half-life of antibodies are crucial for improving the interpretation of these results and for identifying the most useful serological markers of past and recent malaria infection.
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