Evidence map›Paper›PMID 26336928›Full record

ArticleAmerican journal of physiology. Gastrointestinal and liver physiology2015

PACAP intraperitoneal treatment suppresses appetite and food intake via PAC1 receptor in mice by inhibiting ghrelin and increasing GLP-1 and leptin.

John P Vu, Deepinder Goyal, Leon Luong, Suwan Oh, Ravneet Sandhu, Joshua Norris, William Parsons, Joseph R Pisegna, Patrizia M Germano

Registry-linked trialAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in American journal of physiology. Gastrointestinal and liver physiology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. It is linked to trial NCT01146704 (Gastrointestinal Hormonal Regulation of Obesity), which is not on this map. Cited by 23 papers.

0numbers the graph read from it
0cells of the map it votes in
23citing papers in PubMed
0.7field-weighted citation impact, top 35% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

NCT01146704 nacompletednot on this map

Gastrointestinal Hormonal Regulation of Obesity

TypeinterventionalSponsorVA Office of Research and DevelopmentRan2010 to 2019Enrolled31ConditionsObesityArmsProtein powder supplement, standard dosage based on 0.5 gram protein per pound of subject's lean body mass, Protein powder supplement, High Level Protein, based on 1 gram of protein per pound of lean body mass: 25% protein and 45% carbohydrate
3 · Its place in the literature

Who cites it

23 citing papers in PubMed, 36 citations in OpenAlex.

  1. Article
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  5. Mitragynine and morphine produce dose-dependent bimodal action on food but not water intake in rats.American journal of physiology. Regulatory, integrative and comparative physiology · 2024
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  15. PACAP: A regulator of mammalian reproductive function.Molecular and cellular endocrinology · 2020
    Review
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  17. Article
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  20. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 2 institutions in 1 country.

John P VuResearch Service, Veterans Affairs Greater Los Angeles Healthcare System, Los Angeles, California;
Deepinder GoyalDivision of Digestive Diseases, David Geffen School of Medicine, University of California, Los Angeles, California;
Leon LuongMolecular, Cellular & Integrative Physiology Program, University of California, Los Angeles, California;
Suwan OhResearch Service, Veterans Affairs Greater Los Angeles Healthcare System, Los Angeles, California;
Ravneet SandhuResearch Service, Veterans Affairs Greater Los Angeles Healthcare System, Los Angeles, California;
Joshua NorrisResearch Service, Veterans Affairs Greater Los Angeles Healthcare System, Los Angeles, California;
William ParsonsResearch Service, Veterans Affairs Greater Los Angeles Healthcare System, Los Angeles, California; Molecular, Cellular & Integrative Physiology Program, University of California, Los Angeles, California;
Joseph R PisegnaResearch Service, Veterans Affairs Greater Los Angeles Healthcare System, Los Angeles, California; Molecular, Cellular & Integrative Physiology Program, University of California, Los Angeles, California; Division of Gastroenterology, Hepatology and Parenteral Nutrition, Veterans Affairs Greater Los Angeles Healthcare System, Los Angeles, California; CURE/Digestive Diseases Research Center, Department of Medicine, University of California, Los Angeles, California; and.
Patrizia M GermanoResearch Service, Veterans Affairs Greater Los Angeles Healthcare System, Los Angeles, California; CURE/Digestive Diseases Research Center, Department of Medicine, University of California, Los Angeles, California; and Division of Pulmonary and Critical Care, Veterans Affairs Greater Los Angeles Healthcare System, Los Angeles, California pgermano@ucla.edu.
VA Greater Los Angeles Healthcare System · USUniversity of California, Los Angeles · US

Funding

Pilot and Feasibility ProgramP30DK041301 · NIDDK · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI ROZENGURT, JUAN ENRIQUE · 1990 to 2019
$18.0M
Gastrointestinal Hormonal Regulation of ObesityI01RX000194 · VA · VA GREATER LOS ANGELES HEALTHCARE SYSTEM · PI PISEGNA, JOSEPH R, TACHE, YVETTE FRANCE · 2012 to 2015
–
Regulation of Appetite and Obesity by PACAPI01RX000873 · VA · VA GREATER LOS ANGELES HEALTHCARE SYSTEM · PI GERMANO, PATRICIA MARIA · 2015 to 2015
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BLRD VA I01 BX004560Intramural VA VA999999NIDDK NIH HHS P30 DK041301NIDDK NIH HHS P30 DK-41301RRD VA I01 RX000194RRD VA I01 RX000873
6 · The paper itself

Abstract

Pituitary adenylate cyclase-activating peptide (PACAP) is expressed within the gastroenteric system, where it has profound physiological effects. PACAP was shown to regulate food intake and thermogenesis centrally; however, PACAP peripheral regulation of appetite and feeding behavior is unknown. Therefore, we studied PACAP's effect on appetite and food intake control by analyzing feeding behavior and metabolic hormones in PAC1-deficient (PAC1-/-) and age-matched wild-type (WT) mice intraperitoneally injected with PACAP1-38 or PACAP1-27 before the dark phase of feeding. Food intake and feeding behavior were analyzed using the BioDAQ system. Active ghrelin, glucagon-like peptide-1 (GLP-1), leptin, peptide YY, pancreatic polypeptide, and insulin were measured following PACAP1-38 administration in fasted WT mice. PACAP1-38/PACAP1-27 injected into WT mice significantly decreased in a dose-dependent manner cumulative food intake and reduced bout and meal feeding parameters. Conversely, PACAP1-38 injected into PAC1-/- mice failed to significantly change food intake. Importantly, PACAP1-38 reduced plasma levels of active ghrelin compared with vehicle in WT mice. In PAC1-/- mice, fasting levels of active ghrelin, GLP-1, insulin, and leptin and postprandial levels of active ghrelin and insulin were significantly altered compared with levels in WT mice. Therefore, PAC1 is a novel regulator of appetite/satiety. PACAP1-38/PACAP1-27 significantly reduced appetite and food intake through PAC1. In PAC1-/- mice, the regulation of anorexigenic/orexigenic hormones was abolished, whereas active ghrelin remained elevated even postprandially. PACAP significantly reduced active ghrelin in fasting conditions. These results establish a role for PACAP via PAC1 in the peripheral regulation of appetite/satiety and suggest future studies to explore a therapeutic use of PACAP or PAC1 agonists for obesity treatment.

Indexed as

GhrelinPituitary Adenylate Cyclase-Activating PolypeptideAnimalsAppetiteAppetite RegulationDose-Response Relationship, DrugEatingFeeding BehaviorGastrointestinal TractGlucagon-Like Peptide 1Injections, IntraperitonealLeptinMaleMiceModels, AnimalNeurotransmitter AgentsGhrelinGlucagon-Like Peptide 1LeptinNeurotransmitter AgentsPeptide YYPituitary Adenylate Cyclase-Activating PolypeptideReceptors, Pituitary Adenylate Cyclase-Activating Polypeptide, Type IappetiteghrelinGLP-1leptinPAC1 receptorpituitary adenylate cyclase-activating peptide

Identifiers

PMID26336928
PMCPMC4652141
OpenAlexW2125242183

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.