ArticleBMC plant biology2015
Triterpenoid profiling and functional characterization of the initial genes involved in isoprenoid biosynthesis in neem (Azadirachta indica).
Article in BMC plant biology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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15 citing papers in PubMed.
- A comprehensive review ofBiochemistry and biophysics reports · 2026Review
- Comprehensive genome-wide analysis of theFrontiers in plant science · 2025Article
- Meliaceae genomes provide insights into wood development and limonoids biosynthesis.Plant biotechnology journal · 2023Article
- Deciphering the key pathway for triterpenoid biosynthesis inFrontiers in plant science · 2023Review
- Antiparasitic potentiality of ethanol and methanol extracts ofJournal of parasitic diseases : official organ of the Indian Society for Parasitology · 2021Article
- Azadirachta indica MicroRNAs: Genome-Wide Identification, Target Transcript Prediction, and Expression Analyses.Applied biochemistry and biotechnology · 2021Article
- Article
- The MYB transcription factor CiMYB42 regulates limonoids biosynthesis in citrus.BMC plant biology · 2020Article
- Effect of Plant Growth Regulators on Coloured Callus Formation and Accumulation of Azadirachtin, an Essential Biopesticide inPlants (Basel, Switzerland) · 2020Article
- Identification of key enzymes responsible for protolimonoid biosynthesis in plants: Opening the door to azadirachtin production.Proceedings of the National Academy of Sciences of the United States of America · 2019Article
- Tracing the biosynthetic origin of limonoids and their functional groups through stable isotope labeling and inhibition in neem tree (Azadirachta indica) cell suspension.BMC plant biology · 2018Article
- Phytochemical analysis and docking study of compounds present in a polyherbal preparation used in the treatment of dermatophytosis.Current medical mycology · 2017Article
- A homomeric geranyl diphosphate synthase-encoding gene from Camptotheca acuminata and its combinatorial optimization for production of geraniol in Escherichia coli.Journal of industrial microbiology & biotechnology · 2017Article
- Transcriptome and metabolite analyses in Azadirachta indica: identification of genes involved in biosynthesis of bioactive triterpenoids.Scientific reports · 2017Article
- Neem in Dermatology: Shedding Light on the Traditional Panacea.Indian journal of dermatologyArticle
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8 authors.
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Abstract
backgroundNeem tree (Azadirachta indica) is one of the richest sources of skeletally diverse triterpenoids and they are well-known for their broad-spectrum pharmacological and insecticidal properties. However, the abundance of Neem triterpenoids varies among the tissues. Here, we delineate quantitative profiling of fifteen major triterpenoids across various tissues including developmental stages of kernel and pericarp, flower, leaf, stem and bark using UPLC-ESI(+)-HRMS based profiling. Transcriptome analysis was used to identify the initial genes involved in isoprenoid biosynthesis. Based on transcriptome analysis, two short-chain prenyltransferases and squalene synthase (AiSQS) were cloned and functionally characterized.
resultsQuantitative profiling revealed differential abundance of both total and individual triterpenoid content across various tissues. RNA from tissues with high triterpenoid content (fruit, flower and leaf) were pooled to generate 79.08 million paired-end reads using Illumina GA ΙΙ platform. 41,140 transcripts were generated by d e novo assembly. Transcriptome annotation led to the identification of the putative genes involved in isoprenoid biosynthesis. Two short-chain prenyltransferases, geranyl diphosphate synthase (AiGDS) and farnesyl diphosphate synthase (AiFDS) and squalene synthase (AiSQS) were cloned and functionally characterized using transcriptome data. RT-PCR studies indicated five-fold and ten-fold higher relative expression level of AiSQS in fruits as compared to leaves and flowers, respectively.
conclusionsTriterpenoid profiling indicated that there is tissue specific variation in their abundance. The mature seed kernel and initial stages of pericarp were found to contain the highest amount of limonoids. Furthermore, a wide diversity of triterpenoids, especially C-seco triterpenoids were observed in kernel as compared to the other tissues. Pericarp, flower and leaf contained mainly ring-intact triterpenoids. The initial genes such as AiGDS, AiFDS and AiSQS involved in the isoprenoids biosynthesis have been functionally characterized. The expression levels of AiFDS and AiSQS were found to be in correlation with the total triterpenoid content in individual tissues.
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