ArticleToxicon : official journal of the International Society on Toxinology2015
Identification of hyaluronidase and phospholipase B in Lachesis muta rhombeata venom.
Article in Toxicon : official journal of the International Society on Toxinology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 36 citations in OpenAlex.
- Article
- Identification and Characterization of a Highly Active Hyaluronan Lyase fromMarine drugs · 2024Article
- Severe Bushmaster Snakebite Envenoming: Case Report and Overview.Reports (MDPI) · 2024Article
- Paving the way towards effective plant-based inhibitors of hyaluronidase and tyrosinase: a critical review on a structure-activity relationship.Journal of enzyme inhibition and medicinal chemistry · 2022Review
- Action of Varespladib (LY-315920), a Phospholipase AFrontiers in pharmacology · 2021Article
- A brief review on the natural history, venomics and the medical importance of bushmaster (Toxicon: X · 2020Article
- The Sequence and Three-Dimensional Structure Characterization of Snake Venom Phospholipases B.Frontiers in molecular biosciences · 2020Article
- Heterologous expression and mutagenesis of recombinantThe journal of venomous animals and toxins including tropical diseases · 2019Article
- Subproteome ofThe journal of venomous animals and toxins including tropical diseases · 2019Article
- In vitro assessment of cytotoxic activities of Lachesis muta muta snake venom.PLoS neglected tropical diseases · 2018Article
- Purification and enzymatic characterization of a novel metalloprotease fromThe journal of venomous animals and toxins including tropical diseases · 2018Article
- Experimental Lachesis muta rhombeata envenomation and effects of soursop (Annona muricata) as natural antivenom.The journal of venomous animals and toxins including tropical diseases · 2016Article
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Authors and funding
7 authors at 3 institutions in 2 countries.
Funding
Abstract
Hyaluronidases contribute to local and systemic damages after envenoming, since they act as spreading factors cleaving the hyaluronan presents in the connective tissues of the victim, facilitating the diffusion of venom components. Although hyaluronidases are ubiquitous in snake venoms, they still have not been detected in transcriptomic analysis of the Lachesis venom gland and neither in the proteome of its venom performed previously. This work purified a hyaluronidase from Lachesis muta rhombeata venom whose molecular mass was estimated by SDS-PAGE to be 60 kDa. The hyaluronidase was more active at pH 6 and 37 °C when salt concentration was kept constant and more active in the presence of 0.15 M monovalent ions when the pH was kept at 6. Venom was fractionated by reversed-phase liquid chromatography (RPLC). Edman sequencing after RPLC failed to detect hyaluronidase, but identified a new serine proteinase isoform. The hyaluronidase was identified by mass spectrometry analysis of the protein bands in SDS-PAGE. Additionally, phospholipase B was identified for the first time in Lachesis genus venom. The discovery of new bioactive molecules might contribute to the design of novel drugs and biotechnology products as well as to development of more effective treatments against the envenoming.
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