ArticleBMC genomics2015
Large scale RNAi screen in Tribolium reveals novel target genes for pest control and the proteasome as prime target.
Article in BMC genomics, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 71 papers.
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Who cites it
71 citing papers in PubMed, 173 citations in OpenAlex.
- Identification of broadly expressed nervous system-related genes as effective RNAi targets in the brown marmorated stink bug, Halyomorpha halys.Pest management science · 2026Article
- Review
- Mixed Evidence that Dosage Sensitive Genes Drive Global Dosage Compensation in Flour Beetles.Genome biology and evolution · 2026Article
- Emerging experimental and bioinformatic approaches in RNA interference-based pest control research.Insect molecular biology · 2026Review
- Efficacy of Select Gene Targets for the Management of Western Flower Thrips Through RNA Interference.Archives of insect biochemistry and physiology · 2026Article
- A model system test of trophic transmission of RNA interference effects using the milkweed bug Oncopeltus fasciatus (Hemiptera).Scientific reports · 2026Article
- Evaluating the potential of RNA interference for control of striped cucumber beetle, Acalymma vittatum (Fabricius) (Coleoptera: Chrysomelidae).Pest management science · 2026Article
- Microfluidic process-property correlations of dsRNA lipid nanoparticle formulations.Scientific reports · 2026Article
- Contact Unmodified Antisense DNA Biotechnology (CUADb)-Based Oligonucleotide Insecticides and RNA Biocontrols: Molecular Bases and Potential in Plant Protection.Current issues in molecular biology · 2026Review
- Evaluation of dsRNA produced inFrontiers in insect science · 2026Article
- Improving RNAi in the fall armyworm,Frontiers in insect science · 2026Article
- In the Spotlight-Established Researcher.Journal of experimental zoology. Part B, Molecular and developmental evolution · 2025Article
- Article
- The quest for the best target genes for RNAi-mediated pest control.Insect molecular biology · 2025Review
- Effective target genes for RNA interference-based management of the cabbage stem flea beetle.Insect molecular biology · 2025Article
- Review
- Target gene selection for sprayable dsRNA-based biopesticide against Tetranychus urticae Koch (Acari: Tetranychidae).Pest management science · 2025Article
- Superior target genes and pathways for RNAi-mediated pest control revealed by genome-wide analysis in the beetle Tribolium castaneum.Pest management science · 2025Article
- Targeting vacuolar ATPase in tobacco whitefly,Journal of biosciences · 2025Review
- Genome-wide selection of potential target candidates for RNAi against Nilaparvata lugens.BMC genomics · 2024Article
11 more citing papers are in PubMed but not listed here.
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Authors and funding
16 authors at 6 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundInsect pest control is challenged by insecticide resistance and negative impact on ecology and health. One promising pest specific alternative is the generation of transgenic plants, which express double stranded RNAs targeting essential genes of a pest species. Upon feeding, the dsRNA induces gene silencing in the pest resulting in its death. However, the identification of efficient RNAi target genes remains a major challenge as genomic tools and breeding capacity is limited in most pest insects impeding whole-animal-high-throughput-screening.
resultsWe use the red flour beetle Tribolium castaneum as a screening platform in order to identify the most efficient RNAi target genes. From about 5,000 randomly screened genes of the iBeetle RNAi screen we identify 11 novel and highly efficient RNAi targets. Our data allowed us to determine GO term combinations that are predictive for efficient RNAi target genes with proteasomal genes being most predictive. Finally, we show that RNAi target genes do not appear to act synergistically and that protein sequence conservation does not correlate with the number of potential off target sites.
conclusionsOur results will aid the identification of RNAi target genes in many pest species by providing a manageable number of excellent candidate genes to be tested and the proteasome as prime target. Further, the identified GO term combinations will help to identify efficient target genes from organ specific transcriptomes. Our off target analysis is relevant for the sequence selection used in transgenic plants.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.