ArticleNature protocols2015
Live imaging of Tribolium castaneum embryonic development using light-sheet-based fluorescence microscopy.
Article in Nature protocols, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed.
- Sixteen isotropic 3D fluorescence live-imaging datasets of gastrulation in the red flour beetle Tribolium castaneum (Coleoptera: Tenebrionidae).Scientific data · 2026Article
- Fifty high-content light sheet fluorescence microscopy datasets of Tribolium castaneum embryogenesis.Scientific data · 2025Article
- Orthologous genes of the red flour beetle Tribolium castaneum and the vinegar fly Drosophila melanogaster.BMC genomic data · 2025Article
- The cryptonephridial/rectal complex: an evolutionary adaptation for water and ion conservation.Biological reviews of the Cambridge Philosophical Society · 2025Review
- A two-level staging system for the embryonic morphogenesis of the Mediterranean fruit fly (medfly) Ceratitis capitata.PloS one · 2024Article
- Next generation marker-based vector concepts for rapid and unambiguous identification of single and double homozygous transgenic organisms.Biology open · 2023Article
- In toto light sheet fluorescence microscopy live imaging datasets of Ceratitis capitata embryonic development.Scientific data · 2022Article
- A deterministic genotyping workflow reduces waste of transgenic individuals by two-thirds.Scientific reports · 2021Article
- Ultra-thin fluorocarbon foils optimise multiscale imaging of three-dimensional native and optically cleared specimens.Scientific reports · 2019Article
- An ancestral apical brain region contributes to the central complex under the control ofeLife · 2019Article
- Pimpinella anisum essential oil nanoemulsions against Tribolium castaneum-insecticidal activity and mode of action.Environmental science and pollution research international · 2018Article
- Article
- Improving your four-dimensional image: traveling through a decade of light-sheet-based fluorescence microscopy research.Nature protocols · 2017Article
- csiLSFM combines light-sheet fluorescence microscopy and coherent structured illumination for a lateral resolution below 100 nm.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
- Light Sheet-based Fluorescence Microscopy of Living or Fixed and Stained Tribolium castaneum Embryos.Journal of visualized experiments : JoVE · 2017Article
- Non-lethal genotyping of Tribolium castaneum adults using genomic DNA extracted from wing tissue.PloS one · 2017Article
- Adult stem cell lineage tracing and deep tissue imaging.BMB reports · 2015Review
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Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Tribolium castaneum has become an important insect model organism for evolutionary developmental biology, genetics and biotechnology. However, few protocols for live fluorescence imaging of Tribolium have been reported, and little image data is available. Here we provide a protocol for recording the development of Tribolium embryos with light-sheet-based fluorescence microscopy. The protocol can be completed in 4-7 d and provides procedural details for: embryo collection, microscope configuration, embryo preparation and mounting, noninvasive live imaging for up to 120 h along multiple directions, retrieval of the live embryo once imaging is completed, and image data processing, for which exemplary data is provided. Stringent quality control criteria for developmental biology studies are also discussed. Light-sheet-based fluorescence microscopy complements existing toolkits used to study Tribolium development, can be adapted to other insect species, and requires no advanced imaging or sample preparation skills.
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