ArticleJournal of medical entomology2015
Multiplex qRT-PCR for the Detection of Western Equine Encephalomyelitis, St. Louis Encephalitis, and West Nile Viral RNA in Mosquito Pools (Diptera: Culicidae).
Article in Journal of medical entomology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers, 1 of them a synthesis that pooled it.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
25 citing papers in PubMed, 1 synthesis or guideline pooled it, 41 citations in OpenAlex.
- The life cycle of the alphaviruses: From an antiviral perspective.Antiviral research · 2023Pooled it
- From RAMP to Triplex RT-qPCR: Modernizing Arbovirus Surveillance and Confirming the FirstPathogens (Basel, Switzerland) · 2026Article
- Validation of prototype virus inactivation from seven virus families of pandemic potential with a novel low-cost, field-deployable RNA extraction and storage method.Journal of virological methods · 2026Article
- Genomic characterization of a severe West Nile Virus transmission season using a single reaction amplicon sequencing approach.PLoS neglected tropical diseases · 2026Article
- Comparative vector competence of post-2015 St. Louis encephalitis virus inbioRxiv : the preprint server for biology · 2025Article
- Barcoding Quantitative PCR Assay to Distinguish BetweenTropical medicine and infectious disease · 2025Article
- Diagnosis and genomic characterization of the largest western equine encephalitis virus outbreak in Uruguay during 2023-2024.Npj viruses · 2024Article
- Molecular Epidemiology of Western Equine Encephalitis Virus, South America, 2023-2024.Emerging infectious diseases · 2024Article
- Epidemiologic and environmental characterization of the Re-emergence of St. Louis Encephalitis Virus in California, 2015-2020.PLoS neglected tropical diseases · 2022Article
- Effects of ivermectin treatment of backyard chickens on mosquito dynamics and West Nile virus transmission.PLoS neglected tropical diseases · 2022Article
- Increasing Public Health Mosquito Surveillance in Hidalgo County, Texas to Monitor Vector and Arboviral Presence.Pathogens (Basel, Switzerland) · 2021Article
- Isothermal Nucleic Acid Amplification Technologies for the Detection of Equine Viral Pathogens.Animals : an open access journal from MDPI · 2021Review
- West Nile virus in California, 2003-2018: A persistent threat.PLoS neglected tropical diseases · 2020Article
- Movement of St. Louis encephalitis virus in the Western United States, 2014- 2018.PLoS neglected tropical diseases · 2020Article
- Introduction, Spread, and Establishment of West Nile Virus in the Americas.Journal of medical entomology · 2019Review
- Comparative fitness of West Nile virus isolated during California epidemics.PLoS neglected tropical diseases · 2019Article
- Detection of different enteric viruses in children with diarrheal disease: evidence of the high frequency of mixed infections.Access microbiology · 2019Article
- Validation of two multiplex real-time PCR assays based on single nucleotide polymorphisms of the HA1 gene of equine influenza A virus in order to differentiate between clade 1 and clade 2 Florida sublineage isolates.Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc · 2019Article
- Travel-Associated Zika Cases and Threat of Local Transmission during Global Outbreak, California, USA.Emerging infectious diseases · 2018Article
- Scented Sugar Baits Enhance Detection of St. Louis Encephalitis and West Nile Viruses in Mosquitoes in Suburban California.Journal of medical entomology · 2018Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Following the introduction of West Nile virus into California during the summer of 2003, public health and vector control programs expanded surveillance efforts and were in need of diagnostics capable of rapid, sensitive, and specific detection of arbovirus infections of mosquitoes to inform decision support for intervention. Development of a multiplex TaqMan or real-time semiquantitative reverse transcription polymerase chain reaction (RT-PCR) assay in which three virus specific primer-probe sets were used in the same reaction is described herein for the detection of western equine encephalomyelitis, St. Louis encephalitis and West Nile viral RNA. Laboratory validation and field data from 10 transmission seasons are reported. The comparative sensitivity and specificity of this multiplex assay to singleplex RT-PCR as well as an antigen detection (rapid analyte measurement platform) and standard plaque assays indicate this assay to be rapid and useful in providing mosquito infection data to estimate outbreak risk.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.