ArticlePLoS biology2015
The Actin Nucleator Cobl Is Controlled by Calcium and Calmodulin.
Article in PLoS biology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers, 1 of them a synthesis that pooled it.
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Who cites it
36 citing papers in PubMed, 1 synthesis or guideline pooled it, 57 citations in OpenAlex.
- Genetic Variability in Molecular Pathways Implicated in Alzheimer's Disease: A Comprehensive Review.Frontiers in aging neuroscience · 2021Pooled it
- Epigenetic Mechanisms Shaping Spine Regulation: Unveiling the Role of Cytoskeletal Dynamics and Localized Protein Synthesis.Molecular neurobiology · 2025Review
- Nascent dendrite branches initiated by a localized burst of Spire-dependent actin polymerization.Development (Cambridge, England) · 2025Article
- Emerging Adulthood Is Marked by a Cortical Dendritic Arborization Phase Depending on the Actin Nucleator Cobl.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2025Article
- Calcium transients regulate the apical emergence of basally located progenitors during Xenopus skin development.Nature communications · 2025Article
- JMY powers dendritogenesis and is regulated by CaM revealing a general, critical principle in neuromorphogenesis.Communications biology · 2025Article
- Spontaneous Calcium Bursts Organize the Apical Actin Cytoskeleton of Multiciliated Cells.International journal of molecular sciences · 2025Article
- Mitochondria are positioned at dendritic branch induction sites, a process requiring rhotekin2 and syndapin I.Nature communications · 2025Article
- Article
- Genetics of constant and severe pain in the NAPS2 cohort of recurrent acute and chronic pancreatitis patients.The journal of pain · 2025Article
- EHBP1 Is Critically Involved in the Dendritic Arbor Formation and Is Coupled to Factors Promoting Actin Filament Formation.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2024Article
- Membrane shapers from two distinct superfamilies cooperate in the development of neuronal morphology.The Journal of cell biology · 2023Article
- Phase separation of an actin nucleator by junctional microtubules regulates epithelial function.Science advances · 2023Article
- Building the brush border, one microvillus at a time.Current opinion in cell biology · 2023Review
- The p53 and Calcium Regulated Actin Rearrangement in Model Cells.International journal of molecular sciences · 2022Article
- Histone H3.3 K27M chromatin functions implicate a network of neurodevelopmental factors including ASCL1 and NEUROD1 in DIPG.Epigenetics & chromatin · 2022Article
- Poststroke dendritic arbor regrowth requires the actin nucleator Cobl.PLoS biology · 2021Article
- Dendrite tapering actuates a self-organizing signaling circuit for stochastic filopodia initiation in neurons.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- A two-step actin polymerization mechanism drives dendrite branching.Neural development · 2021Article
- Functional interdependence of the actin nucleator Cobl and Cobl-like in dendritic arbor development.eLife · 2021Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Actin nucleation triggers the formation of new actin filaments and has the power to shape cells but requires tight control in order to bring about proper morphologies. The regulation of the members of the novel class of WASP Homology 2 (WH2) domain-based actin nucleators, however, thus far has largely remained elusive. Our study reveals signal cascades and mechanisms regulating Cordon-Bleu (Cobl). Cobl plays some, albeit not fully understood, role in early arborization of neurons and nucleates actin by a mechanism that requires a combination of all three of its actin monomer-binding WH2 domains. Our experiments reveal that Cobl is regulated by Ca2+ and multiple, direct associations of the Ca2+ sensor Calmodulin (CaM). Overexpression analyses and rescue experiments of Cobl loss-of-function phenotypes with Cobl mutants in primary neurons and in tissue slices demonstrated the importance of CaM binding for Cobl's functions. Cobl-induced dendritic branch initiation was preceded by Ca2+ signals and coincided with local F-actin and CaM accumulations. CaM inhibitor studies showed that Cobl-mediated branching is strictly dependent on CaM activity. Mechanistic studies revealed that Ca2+/CaM modulates Cobl's actin binding properties and furthermore promotes Cobl's previously identified interactions with the membrane-shaping F-BAR protein syndapin I, which accumulated with Cobl at nascent dendritic protrusion sites. The findings of our study demonstrate a direct regulation of an actin nucleator by Ca2+/CaM and reveal that the Ca2+/CaM-controlled molecular mechanisms we discovered are crucial for Cobl's cellular functions. By unveiling the means of Cobl regulation and the mechanisms, by which Ca2+/CaM signals directly converge on a cellular effector promoting actin filament formation, our work furthermore sheds light on how local Ca2+ signals steer and power branch initiation during early arborization of nerve cells-a key process in neuronal network formation.
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