ArticlePhotosynthesis research2016
Metabolic engineering of Dunaliella salina for production of ketocarotenoids.
Article in Photosynthesis research, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 75 citations in OpenAlex.
- Metabolic engineering of Dunaliella salina enhances astaxanthin production: combined effects of heterologous enzyme co-expression and dual-stress optimization.World journal of microbiology & biotechnology · 2026Article
- The Mevalonate Pathway: Innovations, Applications, and Challenges in Biotechnology with Emphasis on Fungal Biology.Journal of fungi (Basel, Switzerland) · 2026Review
- Biohybrid Computing with Proteinoids and Algae.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- Metabolic engineering and cultivation strategies for efficient production of fucoxanthin and related carotenoids.Applied microbiology and biotechnology · 2025Review
- Utilization of Microalgae and Duckweed as Sustainable Protein Sources for Food and Feed: Nutritional Potential and Functional Applications.Journal of agricultural and food chemistry · 2025Review
- Characterization of microalgal β-carotene and astaxanthin: exploring their health-promoting properties under the effect of salinity and light intensity.Biotechnology for biofuels and bioproducts · 2025Review
- Genetic engineering of Nannochloropsis oceanica to produce canthaxanthin and ketocarotenoids.Microbial cell factories · 2024Article
- Advances in Genetic Engineering in Improving Photosynthesis and Microalgal Productivity.International journal of molecular sciences · 2023Review
- Microalgal Carotenoids: Therapeutic Application and Latest Approaches to Enhance the Production.Current issues in molecular biology · 2022Review
- Emerging Trends in Genetic Engineering of Microalgae for Commercial Applications.Marine drugs · 2022Review
- Genome-based identification and comparative analysis of enzymes for carotenoid biosynthesis in microalgae.World journal of microbiology & biotechnology · 2021Article
- Anti-Inflammatory and Anticancer Effects of Microalgal Carotenoids.Marine drugs · 2021Review
- Harnessing Solar Energy using Phototrophic Microorganisms: A Sustainable Pathway to Bioenergy, Biomaterials, and Environmental Solutions.Renewable & sustainable energy reviews · 2021Article
- Strategies for enhancing terpenoids accumulation in microalgae.Applied microbiology and biotechnology · 2021Review
- Metabolic Engineering for Carotenoid Production Using Eukaryotic Microalgae and Prokaryotic Cyanobacteria.Advances in experimental medicine and biology · 2021Article
- Metabolic engineering of ketocarotenoids biosynthetic pathway in Chlamydomonas reinhardtii strain CC-4102.Scientific reports · 2020Article
- Bioengineering of Microalgae: Recent Advances, Perspectives, and Regulatory Challenges for Industrial Application.Frontiers in bioengineering and biotechnology · 2020Review
- Article
- The Synthetic Biology Toolkit for Photosynthetic Microorganisms.Plant physiology · 2019Review
- Microalgae for the production of lipid and carotenoids: a review with focus on stress regulation and adaptation.Biotechnology for biofuels · 2018Review
Corrections and comments
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Authors and funding
5 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Dunaliella is a commercially important marine alga producing high amount of β-carotene. The use of Dunaliella as a potential transgenic system for the production of recombinant proteins has been recently recognized. The present study reports for the first time the metabolic engineering of carotenoid biosynthesis in Dunaliella salina for ketocarotenoid production. The pathway modification included the introduction of a bkt gene from H. pluvialis encoding β-carotene ketolase (4,4'β-oxygenase) along with chloroplast targeting for the production of ketocarotenoids. The bkt under the control of Dunaliella Rubisco smaller subunit promoter along with its transit peptide sequence was introduced into the alga through standardized Agrobacterium-mediated transformation procedure. The selected transformants were confirmed using GFP and GUS expression, PCR and southern blot analysis. A notable upregulation of the endogenous hydroxylase level of transformants was observed where the BKT expression was higher in nutrient-limiting conditions. Carotenoid analysis of the transformants through HPLC and MS analysis showed the presence of astaxanthin and canthaxanthin with maximum content of 3.5 and 1.9 µg/g DW, respectively. The present study reports the feasibility of using D. salina for the production of ketocarotenoids including astaxanthin.
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