ArticleScientific reports2015
Droplet Digital Enzyme-Linked Oligonucleotide Hybridization Assay for Absolute RNA Quantification.
Article in Scientific reports, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
13 citing papers in PubMed, 30 citations in OpenAlex.
- Effect of droplet size on the encapsulation efficiency of microparticles in passive microfluidic systems.Biomedical microdevices · 2026Article
- Emerging ctDNA detection strategies in clinical cancer theranostics.Smart medicine · 2023Review
- Recent Advances in Digital Biosensing Technology.Biosensors · 2022Review
- Figure of Merit for CRISPR-Based Nucleic Acid-Sensing Systems: Improvement Strategies and Performance Comparison.ACS sensors · 2022Article
- Microscopic Imaging Methods for Organ-on-a-Chip Platforms.Micromachines · 2022Review
- Combinatorial nanodroplet platform for screening antibiotic combinations.Lab on a chip · 2022Article
- Article
- Droplet-Based Single-Cell Measurements of 16S rRNA Enable Integrated Bacteria Identification and Pheno-Molecular Antimicrobial Susceptibility Testing from Clinical Samples in 30 min.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2021Article
- Diagnosing mild traumatic brain injury using saliva RNA compared to cognitive and balance testing.Clinical and translational medicine · 2020Article
- A microfluidic alternating-pull-push active digitization method for sample-loss-free digital PCR.Lab on a chip · 2019Article
- Droplet microfluidics for high-sensitivity and high-throughput detection and screening of disease biomarkers.Wiley interdisciplinary reviews. Nanomedicine and nanobiotechnology · 2018Review
- Accelerating bacterial growth detection and antimicrobial susceptibility assessment in integrated picoliter droplet platform.Biosensors & bioelectronics · 2017Article
- Analysis of single nucleic acid molecules in micro- and nano-fluidics.Lab on a chip · 2016Review
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
We present a continuous-flow droplet-based digital Enzyme-Linked Oligonucleotide Hybridization Assay (droplet digital ELOHA) for sensitive detection and absolute quantification of RNA molecules. Droplet digital ELOHA incorporates direct hybridization and single enzyme reaction via the formation of single probe-RNA-probe (enzyme) complex on magnetic beads. It enables RNA detection without reverse transcription and PCR amplification processes. The magnetic beads are subsequently encapsulated into a large number of picoliter-sized droplets with enzyme substrates in a continuous-flow device. This device is capable of generating droplets at high-throughput. It also integrates in-line enzymatic incubation and detection of fluorescent products. Our droplet digital ELOHA is able to accurately quantify (differentiate 40% difference) as few as ~600 RNA molecules in a 1 mL sample (equivalent to 1 aM or lower) without molecular replication. The absolute quantification ability of droplet digital ELOHA is demonstrated with the analysis of clinical Neisseria gonorrhoeae 16S rRNA to show its potential value in real complex samples.
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Registered trials
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