ArticlePathogens and disease2015
Pathogenic potential of Escherichia coli clinical strains from orthopedic implant infections towards human osteoblastic cells.
Article in Pathogens and disease, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers, 1 of them a synthesis that pooled it.
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Who cites it
33 citing papers in PubMed, 1 synthesis or guideline pooled it, 56 citations in OpenAlex.
- Antibacterial Titanium Implants Biofunctionalized by Plasma Electrolytic Oxidation with Silver, Zinc, and Copper: A Systematic Review.International journal of molecular sciences · 2021Pooled it
- Review
- DAMPs, PAMPs, NLRs, RIGs, CLRs and TLRs - Understanding the Alphabet Soup in the Context of Bone Biology.Current osteoporosis reports · 2025Review
- Antimicrobial effect of blue light on antibiotic-sensitive and drug-resistant Escherichia coli: a novel isotropic optical fibre.Access microbiology · 2025Article
- Physico-Chemical and Biological Features of Fluorine-Substituted Hydroxyapatite Suspensions.Materials (Basel, Switzerland) · 2024Article
- Multidrug-resistant Shiga toxin-producing Escherichia coli and hybrid pathogenic strains of bovine origin.Veterinary research communications · 2023Article
- Electrostatic and Covalent Binding of an Antibacterial Polymer to Hydroxyapatite for Protection againstMaterials (Basel, Switzerland) · 2023Article
- Characterization and investigation of biological properties of silver nanoparticle-doped hydroxyapatite-based surfaces on zirconium.Scientific reports · 2023Article
- Promising applications of D-amino acids in periprosthetic joint infection.Bone research · 2023Review
- Implant Surfaces Containing Bioglasses and Ciprofloxacin as Platforms for Bone Repair and Improved Resistance to Microbial Colonization.Pharmaceutics · 2022Article
- Bio-Inspired Nanostructured Ti-6Al-4V Alloy: The Role of Two Alkaline Etchants and the Hydrothermal Processing Duration on Antibacterial Activity.Nanomaterials (Basel, Switzerland) · 2022Article
- Role of Implantable Drug Delivery Devices with Dual Platform Capabilities in the Prevention and Treatment of Bacterial Osteomyelitis.Bioengineering (Basel, Switzerland) · 2022Review
- New solutions for combatting implant bacterial infection based on silver nano-dispersed and gallium incorporated phosphate bioactive glass sputtered films: A preliminary study.Bioactive materials · 2022Article
- Long-term antibacterial properties of a nanostructured titanium alloy surface: AnMaterials today. Bio · 2022Article
- Development of Chitosan-Based Surfaces to Prevent Single- and Dual-Species Biofilms ofMolecules (Basel, Switzerland) · 2021Article
- Effect of Gold Nanoparticles and Silicon on the Bioactivity and Antibacterial Properties of Hydroxyapatite/Chitosan/Tricalcium Phosphate-Based Biomicroconcretes.Materials (Basel, Switzerland) · 2021Article
- Dietary flaxseed's protective effects on body tissues of mice after oral exposure to xylene.Saudi journal of biological sciences · 2021Article
- Induced Biological Response in Contact with Ag-and Cu-Doped Carbon Coatings for Potential Orthopedic Applications.Materials (Basel, Switzerland) · 2021Article
- Article
- Nanomaterials-Upconverted Hydroxyapatite for Bone Tissue Engineering and a Platform for Drug Delivery.International journal of nanomedicine · 2021Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
10 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Escherichia coli is one of the first causes of Gram-negative orthopedic implant infections (OII), but little is known about the pathogenicity of this species in such infections that are increasing due to the ageing of the population. We report how this pathogen interacts with human osteoblastic MG-63 cells in vitro, by comparing 20 OII E. coli strains to two Staphylococcus aureus and two Pseudomonas aeruginosa strains. LDH release assay revealed that 6/20 (30%) OII E. coli induced MG-63 cell lysis whereas none of the four control strains was cytotoxic after 4 h of coculture. This high cytotoxicity was associated with hemolytic properties and linked to hlyA gene expression. We further showed by gentamicin protection assay and confocal microscopy that the non-cytotoxic E. coli were not able to invade MG-63 cells unlike S. aureus strains (internalization rate <0.01% for the non-cytotoxic E. coli versus 8.88 ± 2.31% and 4.60 ± 0.42% for both S. aureus). The non-cytotoxic E. coli also demonstrated low adherence rates (<7%), the most adherent E. coli eliciting higher IL-6 and TNF-α mRNA expression in the osteoblastic cells. Either highly cytotoxic or slightly invasive OII E. coli do not show the same infection strategies as S. aureus towards osteoblasts.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.