ArticleNature communications2015
Two-photon-like microscopy with orders-of-magnitude lower illumination intensity via two-step fluorescence.
Article in Nature communications, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
7 citing papers in PubMed.
- Exploiting negative photochromism to harness a four-photon-like fluorescence response with two-photon excitation.Nature communications · 2025Article
- Multiphoton single-molecule localization by sequential excitation with light minima.Light, science & applications · 2022Article
- Photoactivated voltage imaging in tissue with an archaerhodopsin-derived reporter.Science advances · 2021Article
- Toward Two-Photon Absorbing Dyes with Unusually Potentiated Nonlinear Fluorescence Response.Journal of the American Chemical Society · 2020Article
- 3D sub-diffraction imaging in a conventional confocal configuration by exploiting super-linear emitters.Nature communications · 2019Article
- Smart scanning for low-illumination and fast RESOLFT nanoscopy in vivo.Nature communications · 2019Article
- Nonlinear Structured Illumination Using a Fluorescent Protein Activating at the Readout Wavelength.PloS one · 2016Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors.
Funding
Abstract
We describe two-step fluorescence microscopy, a new approach to non-linear imaging based on positive reversible photoswitchable fluorescent probes. The protein Padron approximates ideal two-step fluorescent behaviour: it equilibrates to an inactive state, converts to an active state under blue light, and blue light also excites this active state to fluoresce. Both activation and excitation are linear processes, but the total fluorescent signal is quadratic, proportional to the square of the illumination dose. Here, we use Padron's quadratic non-linearity to demonstrate the principle of two-step microscopy, similar in principle to two-photon microscopy but with orders-of-magnitude better cross-section. As with two-photon, quadratic non-linearity from two-step fluorescence improves resolution and reduces unwanted out-of-focus excitation, and is compatible with structured illumination microscopy. We also show two-step and two-photon imaging can be combined to give quartic non-linearity, further improving imaging in challenging samples. With further improvements, two-step fluorophores could replace conventional fluorophores for many imaging applications.
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Registered trials
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