ArticleBiotechnology and bioengineering2016
Eradication of multidrug-resistant pseudomonas biofilm with pulsed electric fields.
Article in Biotechnology and bioengineering, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers, 1 of them a synthesis that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
27 citing papers in PubMed, 1 synthesis or guideline pooled it, 71 citations in OpenAlex.
- Operational efficiencies and sustainable bioprocessing in electro-fermentation and microbial fuel cells.Bioprocess and biosystems engineering · 2026Pooled it
- A Prospective, Randomized, Controlled Study to Evaluate the Effectiveness of a Fabric-Based Wireless Electroceutical Dressing Compared to Standard-of-Care Treatment Against Acute Trauma and Burn Wound Biofilm Infection.Advances in wound care · 2024Trial
- Magnetic Field-Driven Strategies for Biofilm Disruption: From Iron Oxide Nanoparticles to Adaptive Swarms of Magnetic Microrobots.ACS nano · 2026Review
- Effect of Chemical Sanitizers Alone or Combined with An Enzymatic Product Against Pseudomonas fluorescens Biofilms on Food-Grade Materials.Current microbiology · 2025Article
- Microbubble-Controlled Delivery of Biofilm-Targeting Nanoparticles to Treat MRSA Infection.Advanced functional materials · 2025Article
- Microbiome modulation of implant-related infection by a novel miniaturized pulsed electromagnetic field device.NPJ biofilms and microbiomes · 2025Article
- Cold Plasma Activity Against Biofilm Formation of Prosthetic Joint Infection Pathogens.Pathogens (Basel, Switzerland) · 2024Article
- Comparison of the efficacy of physical and chemical strategies for the inactivation of biofilm cells of foodborne pathogens.Food science and biotechnology · 2023Review
- Photodynamic Therapy, Probiotics, Acetic Acid, and Essential Oil in the Treatment of Chronic Wounds Infected withPharmaceutics · 2023Review
- Biofilms: Formation, Research Models, Potential Targets, and Methods for Prevention and Treatment.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2022Review
- Antimicrobial photodynamic therapy (aPDT) for biofilm treatments. Possible synergy between aPDT and pulsed electric fields.Virulence · 2021Review
- High-Voltage, Pulsed Electric Fields EliminateAdvances in wound care · 2021Article
- Resuscitation of Pulsed Electric Field-TreatedFoods (Basel, Switzerland) · 2021Article
- Eradication ofMicroorganisms · 2020Article
- Electroactive 3D Printed Scaffolds Based on Percolated Composites of Polycaprolactone With Thermally Reduced Graphene Oxide for Antibacterial and Tissue Engineering Applications.Nanomaterials (Basel, Switzerland) · 2020Article
- What Are the Effects of Irreversible Electroporation on a Staphylococcus aureus Rabbit Model of Osteomyelitis?Clinical orthopaedics and related research · 2019Article
- Biofilm Bridges Forming Structural Networks on Patterned Lubricant-Infused Surfaces.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2019Article
- Low concentrations of acetic and formic acids enhance the inactivation of Staphylococcus aureus and Pseudomonas aeruginosa with pulsed electric fields.BMC microbiology · 2019Article
- Selective Inactivation ofAdvances in wound care · 2019Article
- Electroactive Smart Polymers for Biomedical Applications.Materials (Basel, Switzerland) · 2019Review
Corrections and comments
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Authors and funding
8 authors at 4 institutions in 2 countries.
Funding
Abstract
Biofilm formation is a significant problem, accounting for over eighty percent of microbial infections in the body. Biofilm eradication is problematic due to increased resistance to antibiotics and antimicrobials as compared to planktonic cells. The purpose of this study was to investigate the effect of Pulsed Electric Fields (PEF) on biofilm-infected mesh. Prolene mesh was infected with bioluminescent Pseudomonas aeruginosa and treated with PEF using a concentric electrode system to derive, in a single experiment, the critical electric field strength needed to kill bacteria. The effect of the electric field strength and the number of pulses (with a fixed pulse length duration and frequency) on bacterial eradication was investigated. For all experiments, biofilm formation and disruption were confirmed with bioluminescent imaging and Scanning Electron Microscopy (SEM). Computation and statistical methods were used to analyze treatment efficiency and to compare it to existing theoretical models. In all experiments 1500 V are applied through a central electrode, with pulse duration of 50 μs, and pulse delivery frequency of 2 Hz. We found that the critical electric field strength (Ecr) needed to eradicate 100-80% of bacteria in the treated area was 121 ± 14 V/mm when 300 pulses were applied, and 235 ± 6.1 V/mm when 150 pulses were applied. The area at which 100-80% of bacteria were eradicated was 50.5 ± 9.9 mm(2) for 300 pulses, and 13.4 ± 0.65 mm(2) for 150 pulses. 80% threshold eradication was not achieved with 100 pulses. The results indicate that increased efficacy of treatment is due to increased number of pulses delivered. In addition, we that showed the bacterial death rate as a function of the electrical field follows the statistical Weibull model for 150 and 300 pulses. We hypothesize that in the clinical setting, combining systemic antibacterial therapy with PEF will yield a synergistic effect leading to improved eradication of mesh infections.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.