Evidence map›Paper›PMID 26332284›Full record

ArticleACS synthetic biology2016

A Microfluidic Platform for Long-Term Monitoring of Algae in a Dynamic Environment.

Chung Sze Luke, Jangir Selimkhanov, Leo Baumgart, Susan E Cohen, Susan S Golden, Natalie A Cookson, Jeff Hasty

Abstract read
In one paragraph

Article in ACS synthetic biology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.

0numbers the graph read from it
0cells of the map it votes in
16citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

16 citing papers in PubMed.

  1. Uncoupling growth and division inISME communications · 2025
    Article
  2. Article
  3. Article
  4. Article
  5. Microfluidic Microalgae System: A Review.Molecules (Basel, Switzerland) · 2022
    Review
  6. Article
  7. Review
  8. Article
  9. Review
  10. Article
  11. Article
  12. Microfluidic technology for plankton research.Current opinion in biotechnology · 2019
    Review
  13. Article
  14. Article
  15. Review
  16. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Chung Sze LukeDepartment of Bioengineering, University of California, San Diego , La Jolla, California 92093, United States.
Jangir SelimkhanovDepartment of Bioengineering, University of California, San Diego , La Jolla, California 92093, United States.
Leo BaumgartDivision of Biological Sciences, University of California, San Diego , La Jolla, California 92093, United States.
Susan E CohenDivision of Biological Sciences, University of California, San Diego , La Jolla, California 92093, United States.
Susan S GoldenDivision of Biological Sciences, University of California, San Diego , La Jolla, California 92093, United States.
Natalie A CooksonBioCircuits Institute, University of California, San Diego , La Jolla, California 92093, United States.
Jeff HastyDepartment of Bioengineering, University of California, San Diego , La Jolla, California 92093, United States.

Funding

Engineered Gene Circuits for Basic Science and BiotechnologyR01GM069811 · NIGMS · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI HASTY, JEFF M, TSIMRING, LEV S · 2004 to 2025
$13.6M
Circadian gating of cell division by the cyanobacterial oscillatorR01GM100116 · NIGMS · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI GOLDEN, SUSAN S, POGLIANO, JOSEPH A · 2012 to 2015
$1.5M
NIGMS NIH HHS GM100116NIGMS NIH HHS R01 GM069811NIGMS NIH HHS R01 GM100116NIGMS NIH HHS R01GM69811
6 · The paper itself

Abstract

Culturing cells in microfluidic "lab-on-a-chip" devices for time lapse microscopy has become a valuable tool for studying the dynamics of biological systems. Although microfluidic technology has been applied to culturing and monitoring a diverse range of bacterial and eukaryotic species, cyanobacteria and eukaryotic microalgae present several challenges that have made them difficult to culture in a microfluidic setting. Here, we present a customizable device for the long-term culturing and imaging of three well characterized strains of cyanobacteria and microalgae. This platform has several advantages over agarose pads and demonstrates great potential for obtaining high quality, single-cell gene expression data of cyanobacteria and algae in precisely controlled, dynamic environments over long time periods.

Indexed as

EnvironmentAlgorithmsCell TrackingChlorophyllFluorescenceMicrofluidicsMicroscopy, FluorescenceSynechocystisTime-Lapse ImagingChlorophyllChlorellacyanobacteriadynamicsmicrofluidicsSynechococcusSynechocystis

Identifiers

PMID26332284
PMCPMC5249263

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.