ArticleCurrent protocols in cell biology2015
Supported Lipid Bilayer Technology for the Study of Cellular Interfaces.
Article in Current protocols in cell biology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
14 citing papers in PubMed, 22 citations in OpenAlex.
- Protocol for total internal reflection fluorescence microscopy image preprocessing and radial analysis of LFA-1 in T cell immune synapses.STAR protocols · 2026Article
- Actin waves guide an outward movement of microclusters in the lymphocyte immunological synapse.EMBO reports · 2026Article
- Article
- TIGIT can inhibit T cell activation via ligation-induced nanoclusters, independent of CD226 co-stimulation.Nature communications · 2023Article
- Micropatterning of functional lipid bilayer assays for quantitative bioanalysis.Biomicrofluidics · 2023Article
- Tear Film Stability as a Function of Tunable Mucin Concentration Attached to Supported Lipid Bilayers.The journal of physical chemistry. B · 2022Article
- Article
- Heterogeneity in extracellular vesicle secretion by single human macrophages revealed by super-resolution microscopy.Journal of extracellular vesicles · 2022Article
- Reconstitution of Membrane-associated Components of a G-protein Signaling Pathway on Membrane-coated Nanoparticles (Lipobeads).Bio-protocol · 2022Article
- Synaptic secretion from human natural killer cells is diverse and includes supramolecular attack particles.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- Impact of Nanoscale Hindrances on the Relationship between Lipid Packing and Diffusion in Model Membranes.The journal of physical chemistry. B · 2020Article
- Article
- Contractile actomyosin arcs promote the activation of primary mouse T cells in a ligand-dependent manner.PloS one · 2017Article
- Formin-generated actomyosin arcs propel T cell receptor microcluster movement at the immune synapse.The Journal of cell biology · 2016Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 2 institutions in 1 country.
Funding
Abstract
Glass-supported lipid bilayers presenting freely diffusing proteins have served as a powerful tool for studying cell-cell interfaces, in particular, T cell-antigen presenting cell (APC) interactions, using optical microscopy. Here we expand upon existing protocols and describe the preparation of liposomes by an extrusion method, and describe how this system can be used to study immune synapse formation by Jurkat cells. We also present a method for forming such lipid bilayers on silica beads for the study of signaling responses by population methods, such as western blotting, flow cytometry, and gene-expression analysis. Finally, we describe how to design and prepare transmembrane-anchored protein-laden liposomes, following expression in suspension CHO (CHOs) cells, a mammalian expression system alternative to insect and bacterial cell lines, which do not produce mammalian glycosylation patterns. Such transmembrane-anchored proteins may have many novel applications in cell biology and immunology.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.