ArticleBiomacromolecules2015
Reactive Self-Assembly of Polymers and Proteins to Reversibly Silence a Killer Protein.
Article in Biomacromolecules, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
16 citing papers in PubMed, 36 citations in OpenAlex.
- Polymers of functionalized diaminopropionic acid are efficient mediators of active exogenous enzyme delivery into cells.Scientific reports · 2024Article
- Intracellular delivery of therapeutic proteins. New advancements and future directions.Frontiers in bioengineering and biotechnology · 2023Review
- Exogenous Introduction of Initiator and Executioner Caspases Results in Different Apoptotic Outcomes.JACS Au · 2021Article
- Evaluating Endosomal Escape of Caspase-3-Containing Nanomaterials Using Split GFP.Biomacromolecules · 2021Article
- Tracking exogenous intracellular casp-3 using split GFP.Protein science : a publication of the Protein Society · 2021Article
- Disulfide-containing Macromolecules for Therapeutic Delivery.Israel journal of chemistry · 2020Article
- Reversible Click Chemistry for Ultrafast and Quantitative Formation of Protein-Polymer Nanoassembly and Intracellular Protein Delivery.ACS nano · 2019Article
- Biodistribution Analysis of NIR-Labeled Nanogels Using in Vivo FMT Imaging in Triple Negative Human Mammary Carcinoma Models.Molecular pharmaceutics · 2018Article
- Lipogels for Encapsulation of Hydrophilic Proteins and Hydrophobic Small Molecules.Biomacromolecules · 2018Article
- Utilizing Inverse Emulsion Polymerization To Generate Responsive Nanogels for Cytosolic Protein Delivery.Molecular pharmaceutics · 2017Article
- Article
- Templated Self-Assembly of a Covalent Polymer Network for Intracellular Protein Delivery and Traceless Release.Journal of the American Chemical Society · 2017Article
- Intracellular delivery of proteins by nanocarriers.Nanomedicine (London, England) · 2017Review
- Dual Site Phosphorylation of Caspase-7 by PAK2 Blocks Apoptotic Activity by Two Distinct Mechanisms.Structure (London, England : 1993) · 2017Article
- Nano-Armoring of Enzymes: Rational Design of Polymer-Wrapped Enzymes.Methods in enzymology · 2017Article
- Reprogramming Caspase-7 Specificity by Regio-Specific Mutations and Selection Provides Alternate Solutions for Substrate Recognition.ACS chemical biology · 2016Article
Corrections and comments
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
Abstract
Conjugation of biologically active proteins to polymeric materials is of great interest in the treatment of cancer and other diseases of protein deficiency. The conjugation of such biomacromolecules is challenging both due to their hydrophilicity and propensity to denature under non-native conditions. We describe a novel reactive self-assembly approach to "wrap" a protein with polymers, simultaneously protecting its delicate folded state and silencing its enzymatic activity. This approach has been demonstrated using caspase-3, an apoptosis-inducing protein, as the first case study. The protein-polymer conjugation is designed to be reversed under the native conditions for caspase-3, that is, the reducing environment found in the cytosol. The current strategy allowed release and recovery of up to 86% of caspase activity and nanogel-caspase-3 conjugates induced 70-80% apoptotic cell death shortly thereafter. This approach is widely generalizable and should be applicable to the intracellular delivery of a wide range of therapeutic proteins for treatment of complex and genetic diseases.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.