ArticleNature2015
Computational design of co-assembling protein-DNA nanowires.
Article in Nature, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
What it found
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
29 citing papers in PubMed, 85 citations in OpenAlex.
- Construction of synthetic protein-binding non-genetic DNA systems in living cells.Nature chemistry · 2026Article
- Modulation of Proteinoid Electrical Spiking Activity with Magnetic Nanoparticles.Langmuir : the ACS journal of surfaces and colloids · 2025Article
- Programming crystallization kinetics of self-assembled DNA crystals with 5-methylcytosine modification.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Agglomeration: when folded proteins clump together.Biophysical reviews · 2023Review
- A Colorimetric/Fluorescent Dual-Mode Aptasensor forFoods (Basel, Switzerland) · 2023Article
- Article
- Article
- Assessing and enhancing foldability in designed proteins.Protein science : a publication of the Protein Society · 2022Article
- Helicity-driven chiral self-sorting supramolecular polymerization with AgChemical science · 2022Article
- Nanoscale Structures and Materials from the Self-assembly of Polypeptides and DNA.Current topics in medicinal chemistry · 2022Review
- Protein Assembly by Design.Chemical reviews · 2021Review
- Redefining Protein Interfaces within Protein Single Crystals with DNA.Journal of the American Chemical Society · 2021Article
- Strategies to Build Hybrid Protein-DNA Nanostructures.Nanomaterials (Basel, Switzerland) · 2021Review
- Supramolecular Architectures of Nucleic Acid/Peptide Hybrids.International journal of molecular sciences · 2020Review
- Sequence-based prediction of protein binding mode landscapes.PLoS computational biology · 2020Article
- Article
- Eukaryotic transcription factors can track and control their target genes using DNA antennas.Nature communications · 2020Article
- Hybrid gold/DNA nanowire circuit with sub-10 nm nanostructure arrays.Microsystems & nanoengineering · 2020Article
- Diverse protein assembly driven by metal and chelating amino acids with selectivity and tunability.Nature communications · 2019Article
- Nanowired Bioelectric Interfaces.Chemical reviews · 2019Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Biomolecular self-assemblies are of great interest to nanotechnologists because of their functional versatility and their biocompatibility. Over the past decade, sophisticated single-component nanostructures composed exclusively of nucleic acids, peptides and proteins have been reported, and these nanostructures have been used in a wide range of applications, from drug delivery to molecular computing. Despite these successes, the development of hybrid co-assemblies of nucleic acids and proteins has remained elusive. Here we use computational protein design to create a protein-DNA co-assembling nanomaterial whose assembly is driven via non-covalent interactions. To achieve this, a homodimerization interface is engineered onto the Drosophila Engrailed homeodomain (ENH), allowing the dimerized protein complex to bind to two double-stranded DNA (dsDNA) molecules. By varying the arrangement of protein-binding sites on the dsDNA, an irregular bulk nanoparticle or a nanowire with single-molecule width can be spontaneously formed by mixing the protein and dsDNA building blocks. We characterize the protein-DNA nanowire using fluorescence microscopy, atomic force microscopy and X-ray crystallography, confirming that the nanowire is formed via the proposed mechanism. This work lays the foundation for the development of new classes of protein-DNA hybrid materials. Further applications can be explored by incorporating DNA origami, DNA aptamers and/or peptide epitopes into the protein-DNA framework presented here.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.