Evidence map›Paper›PMID 26330540›Full record

ArticleMolecular & cellular proteomics : MCP2015

Heterogeneous Ribonucleoprotein K (hnRNP K) Binds miR-122, a Mature Liver-Specific MicroRNA Required for Hepatitis C Virus Replication.

Baochang Fan, F X Reymond Sutandy, Guan-Da Syu, Stefani Middleton, Guanghui Yi, Kuan-Yi Lu, Chien-Sheng Chen, C Cheng Kao

Open access · hybridAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Molecular & cellular proteomics : MCP, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.

0numbers the graph read from it
0cells of the map it votes in
19citing papers in PubMed
0.6field-weighted citation impact, top 30% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

19 citing papers in PubMed, 36 citations in OpenAlex.

  1. Article
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  3. Differential miRNA signatures in Hepatitis E Virus Infection: Insights into acute, chronic, and pregnancy-related outcomes.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2025
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 2 institutions in 2 countries.

Baochang FanFrom the ‡Department of Molecular & Cellular Biochemistry, Indiana University, Bloomington, IN 47405, USA;
F X Reymond Sutandy§Graduate Institute of Systems Biology and Bioinformatics, National Central University, Jhongli 32001, Taiwan.
Guan-Da Syu§Graduate Institute of Systems Biology and Bioinformatics, National Central University, Jhongli 32001, Taiwan.
Stefani MiddletonFrom the ‡Department of Molecular & Cellular Biochemistry, Indiana University, Bloomington, IN 47405, USA;
Guanghui YiFrom the ‡Department of Molecular & Cellular Biochemistry, Indiana University, Bloomington, IN 47405, USA;
Kuan-Yi Lu§Graduate Institute of Systems Biology and Bioinformatics, National Central University, Jhongli 32001, Taiwan.
Chien-Sheng Chen§Graduate Institute of Systems Biology and Bioinformatics, National Central University, Jhongli 32001, Taiwan ckao@indiana.edu cchen103@gmail.com.
C Cheng KaoFrom the ‡Department of Molecular & Cellular Biochemistry, Indiana University, Bloomington, IN 47405, USA; ckao@indiana.edu cchen103@gmail.com.
National Central University · TWIndiana University Bloomington · US

Funding

RNA-dependent RNA Synthesis by the Hepatitis C VirusR01AI073335 · NIAID · TRUSTEES OF INDIANA UNIVERSITY · PI KAO, CHENG C · 2008 to 2012
$1.5M
Proteome-wide identification of RNA-binding proteinsR21AI075015 · NIAID · TRUSTEES OF INDIANA UNIVERSITY · PI KAO, CHENG C · 2009 to 2010
$444k
NIAID NIH HHS AI073335NIAID NIH HHS R01 AI073335NIAID NIH HHS R21 AI075015PHS HHS RAI075015A
6 · The paper itself

Abstract

Heterogeneous ribonucleoprotein K (hnRNP K) binds to the 5' untranslated region of the hepatitis C virus (HCV) and is required for HCV RNA replication. The hnRNP K binding site on HCV RNA overlaps with the sequence recognized by the liver-specific microRNA, miR-122. A proteome chip containing ∼17,000 unique human proteins probed with miR-122 identified hnRNP K as one of the strong binding proteins. In vitro kinetic study showed hnRNP K binds miR-122 with a nanomolar dissociation constant, in which the short pyrimidine-rich residues in the central and 3' portion of the miR-122 were required for hnRNP K binding. In liver hepatocytes, miR-122 formed a coprecipitable complex with hnRNP K. High throughput Illumina DNA sequencing of the RNAs precipitated with hnRNP K was enriched for mature miR-122. SiRNA knockdown of hnRNP K in human hepatocytes reduced the levels of miR-122. These results show that hnRNP K is a cellular protein that binds and affects the accumulation of miR-122. Its ability to also bind HCV RNA near the miR-122 binding site suggests a role for miR-122 recognition of HCV RNA.

Indexed as

Binding SitesGene Expression ProfilingGene Expression RegulationGene OntologyHepacivirusHepatocytesHeterogeneous-Nuclear Ribonucleoprotein KHigh-Throughput Nucleotide SequencingHost-Pathogen InteractionsHumansLiverMicroRNAsMolecular Sequence AnnotationProtein Array AnalysisProtein BindingRNA, Small InterferingHeterogeneous-Nuclear Ribonucleoprotein KMicroRNAsMIRN122 microRNA, humanRNA, Small InterferingRNA, Viral

Identifiers

PMID26330540
PMCPMC4638032
OpenAlexW2172649799

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.