ArticlePhysiological genomics2015
Identifying growth hormone-regulated enhancers in the Igf1 locus.
Article in Physiological genomics, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 18 citations in OpenAlex.
- In ovo feeding of methionine affects antioxidant status and growth-related gene expression of TETRA SL and Hungarian indigenous chicks.Scientific reports · 2024Article
- Article
- Plasma Growth Hormone Pulses Induce Male-biased Pulsatile Chromatin Opening and Epigenetic Regulation in Adult Mouse Liver.bioRxiv : the preprint server for biology · 2023Article
- CircAgtpbp1 Acts as a Molecular Sponge of miR-543-5p to Regulate the Secretion of GH in Rat Pituitary Cells.Animals : an open access journal from MDPI · 2021Article
- Regulation of gene expression by growth hormone.Molecular and cellular endocrinology · 2020Review
- Characterizing the complexity of Australian marsupial insulin-like growth factor 1 genes.Molecular and cellular endocrinology · 2019Article
- Roles of differential expression of miR-543-5p in GH regulation in rat anterior pituitary cells and GH3 cells.PloS one · 2019Article
- Insulinlike Growth Factor 1 Gene Variation in Vertebrates.Endocrinology · 2018Article
- The New Genomics: What Molecular Databases Can Tell Us About Human Population Variation and Endocrine Disease.Endocrinology · 2017Review
- Activation of Male Liver Chromatin Accessibility and STAT5-Dependent Gene Transcription by Plasma Growth Hormone Pulses.Endocrinology · 2017Article
- Variation in the Insulin-Like Growth Factor 1 Gene in Primates.Endocrinology · 2017Article
- Article
- Defining human insulin-like growth factor I gene regulation.American journal of physiology. Endocrinology and metabolism · 2016Article
- Human growth hormone and human prolactin function as autocrine/paracrine promoters of progression of hepatocellular carcinoma.Oncotarget · 2016Article
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
Abstract
Growth hormone (GH) plays a central role in regulating somatic growth and in controlling multiple physiological processes in humans and other vertebrates. A key agent in many GH actions is the secreted peptide, IGF-I. As established previously, GH stimulates IGF-I gene expression via the Stat5b transcription factor, leading to production of IGF-I mRNAs and proteins. However, the precise mechanisms by which GH-activated Stat5b promotes IGF-I gene transcription have not been defined. Unlike other GH-regulated genes, there are no Stat5b sites near either of the two IGF-I gene promoters. Although dispersed GH-activated Stat5b binding elements have been mapped in rodent Igf1 gene chromatin, it is unknown how these distal sites might function as potential transcriptional enhancers. Here we have addressed mechanisms of regulation of IGF-I gene transcription by GH by generating cell lines in which the rat Igf1 chromosomal locus has been incorporated into the mouse genome. Using these cells we find that physiological levels of GH rapidly and potently activate Igf1 gene transcription while stimulating physical interactions in chromatin between inducible Stat5b-binding elements and the Igf1 promoters. We have thus developed a robust experimental platform for elucidating how dispersed transcriptional enhancers control Igf1 gene expression under different biological conditions.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.